[RASMB] capillary viscosimeter

[Stephen Harding]

Dear Holger/Sabine/ all.
We looked at Anton-Paar's rolling ball viscometer about 7 years ago and found that silanization of the capillary led to considerable improvement: Anton-Paar - who were really helpful - did this for us on request and I think they still do this, although there is a charge.  [nb. We found standard Ostwald viscometers are still considerably more accurate than the rolling ball for dilute solution intrinsic viscosity work].
My student Yanling Lu wrote up a section on this in her PhD thesis, I will see if I can find it and send it on,
All best
Steve

**********************************************************
Stephen E. Harding, http://www.nottingham.ac.uk/Biosciences/People/steve.harding
Professor of Applied Biochemistry, University of Nottingham
E-post: steve.harding at nottingham.ac.uk<mailto:steve.harding at nottingham.ac.uk>
Tel: +44(0) 78110 90635

From: rasmb-bounces at list.rasmb.org [mailto:rasmb-bounces at list.rasmb.org] On Behalf Of Sabine Kaltofen
Sent: 19 August 2013 13:02
To: HGSR (Holger Martin Strauss); rasmb at list.rasmb.org
Subject: Re: [RASMB] capillary viscosimeter

Hi Holger and all,

have you thought of coating the capillary with a silanizing agant such as SigmaCote that is used in crystallography? It works fine for quartz cuvettes and forms a fairly stable film (you have to boil it in detergent to remove it). I guess you have to recalibrate your capillary, though.

Best
Sabine

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Sabine Kaltofen

Biochemistry & Structural Biology
Lund University
+46 462220851
sabine.kaltofen at biochemistry.lu.se<mailto:sabine.kaltofen at biochemistry.lu.se>
________________________________
From: rasmb-bounces at list.rasmb.org [rasmb-bounces at list.rasmb.org] on behalf of HGSR (Holger Martin Strauss) [hgsr at novonordisk.com]
Sent: 19 August 2013 10:46
To: rasmb at list.rasmb.org
Subject: [RASMB] capillary viscosimeter
Hello everyone,

We've recently ventured into measuring viscosities. We use a capillary viscosimeter Lovis 2000 ME from Anton Paar and have observed several times that high concentration protein solutions stick to the (glass) capillary, after rinsing and scrubbing. We then treat the capillary with piranha solution. This is annoying and cumbersome and we haven't yet found a way to avoid/reduce of protein sticking to the surface. But maybe someone of you has?

Best wishes, Holger

_______________________________

Holger Martin Strauss, PhD.
Senior Scientist
Biophysics

Novo Nordisk A/S
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DK-2760 Måløv
Denmark
+45 4444 8888 (phone)
­+45 30 75 66 94 (mobile)
hgsr at novonordisk.com<mailto:hgsr at novonordisk.com>

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