<html xmlns:v="urn:schemas-microsoft-com:vml" xmlns:o="urn:schemas-microsoft-com:office:office" xmlns:w="urn:schemas-microsoft-com:office:word" xmlns:m="http://schemas.microsoft.com/office/2004/12/omml" xmlns="http://www.w3.org/TR/REC-html40"><head><meta http-equiv=Content-Type content="text/html; charset=iso-8859-1"><meta name=Generator content="Microsoft Word 14 (filtered medium)"><!--[if !mso]><style>v\:* {behavior:url(#default#VML);}
o\:* {behavior:url(#default#VML);}
w\:* {behavior:url(#default#VML);}
.shape {behavior:url(#default#VML);}
</style><![endif]--><style><!--
/* Font Definitions */
@font-face
{font-family:"Cambria Math";
panose-1:2 4 5 3 5 4 6 3 2 4;}
@font-face
{font-family:Calibri;
panose-1:2 15 5 2 2 2 4 3 2 4;}
@font-face
{font-family:Tahoma;
panose-1:2 11 6 4 3 5 4 4 2 4;}
@font-face
{font-family:Verdana;
panose-1:2 11 6 4 3 5 4 4 2 4;}
/* Style Definitions */
p.MsoNormal, li.MsoNormal, div.MsoNormal
{margin:0cm;
margin-bottom:.0001pt;
font-size:11.0pt;
font-family:"Calibri","sans-serif";}
a:link, span.MsoHyperlink
{mso-style-priority:99;
color:blue;
text-decoration:underline;}
a:visited, span.MsoHyperlinkFollowed
{mso-style-priority:99;
color:purple;
text-decoration:underline;}
p
{mso-style-priority:99;
margin:0cm;
margin-bottom:.0001pt;
font-size:12.0pt;
font-family:"Times New Roman","serif";}
p.MsoAcetate, li.MsoAcetate, div.MsoAcetate
{mso-style-priority:99;
mso-style-link:"Balloon Text Char";
margin:0cm;
margin-bottom:.0001pt;
font-size:8.0pt;
font-family:"Tahoma","sans-serif";}
p.msochpdefault, li.msochpdefault, div.msochpdefault
{mso-style-name:msochpdefault;
margin:0cm;
margin-bottom:.0001pt;
font-size:12.0pt;
font-family:"Calibri","sans-serif";}
span.emailstyle17
{mso-style-name:emailstyle17;
font-family:"Calibri","sans-serif";
color:windowtext;}
span.emailstyle19
{mso-style-name:emailstyle19;
font-family:"Verdana","sans-serif";
color:#3F31F7;
font-weight:normal;
font-style:normal;}
span.apple-style-span
{mso-style-name:apple-style-span;}
span.BalloonTextChar
{mso-style-name:"Balloon Text Char";
mso-style-priority:99;
mso-style-link:"Balloon Text";
font-family:"Tahoma","sans-serif";}
span.EmailStyle24
{mso-style-type:personal-reply;
font-family:"Verdana","sans-serif";
color:black;}
.MsoChpDefault
{mso-style-type:export-only;
font-size:10.0pt;
font-family:"Calibri","sans-serif";}
@page WordSection1
{size:612.0pt 792.0pt;
margin:72.0pt 72.0pt 72.0pt 72.0pt;}
div.WordSection1
{page:WordSection1;}
--></style><!--[if gte mso 9]><xml>
<o:shapedefaults v:ext="edit" spidmax="1026" />
</xml><![endif]--><!--[if gte mso 9]><xml>
<o:shapelayout v:ext="edit">
<o:idmap v:ext="edit" data="1" />
</o:shapelayout></xml><![endif]--></head><body lang=EN-GB link=blue vlink=purple><div class=WordSection1><p class=MsoNormal><span style='font-size:10.0pt;font-family:"Verdana","sans-serif";color:black'>Dear Holger/Sabine/ all.<o:p></o:p></span></p><p class=MsoNormal><span style='font-size:10.0pt;font-family:"Verdana","sans-serif";color:black'>We looked at Anton-Paar’s rolling ball viscometer about 7 years ago and found that silanization of the capillary led to considerable improvement: Anton-Paar – who were really helpful - did this for us on request and I think they still do this, although there is a charge. [nb. We found standard Ostwald viscometers are still considerably more accurate than the rolling ball for dilute solution intrinsic viscosity work].<o:p></o:p></span></p><p class=MsoNormal><span style='font-size:10.0pt;font-family:"Verdana","sans-serif";color:black'>My student Yanling Lu wrote up a section on this in her PhD thesis, I will see if I can find it and send it on,<o:p></o:p></span></p><p class=MsoNormal><span style='font-size:10.0pt;font-family:"Verdana","sans-serif";color:black'>All best<o:p></o:p></span></p><p class=MsoNormal><span style='font-size:10.0pt;font-family:"Verdana","sans-serif";color:black'>Steve<o:p></o:p></span></p><p class=MsoNormal><span style='font-size:10.0pt;font-family:"Verdana","sans-serif";color:black'><o:p> </o:p></span></p><div><p class=MsoNormal><i><span lang=DE-CH style='font-size:8.0pt;font-family:"Verdana","sans-serif";color:black'>**********************************************************</span></i><span lang=DE-CH style='font-size:8.0pt;font-family:"Verdana","sans-serif";color:black'><o:p></o:p></span></p><p class=MsoNormal><span lang=DE-CH style='font-size:8.0pt;font-family:"Verdana","sans-serif";color:black'>Stephen E. Harding, <a href="http://www.nottingham.ac.uk/Biosciences/People/steve.harding"><span style='color:black'>http://www.nottingham.ac.uk/Biosciences/People/steve.harding</span></a><br>Professor of Applied Biochemistry, University of Nottingham<br>E-post: <a href="mailto:steve.harding@nottingham.ac.uk"><span style='color:black'>steve.harding@nottingham.ac.uk</span></a><o:p></o:p></span></p><p class=MsoNormal><span lang=DE-CH style='font-size:8.0pt;font-family:"Verdana","sans-serif";color:black'>Tel: +44(0) 78110 90635<o:p></o:p></span></p></div><p class=MsoNormal><span style='font-size:10.0pt;font-family:"Verdana","sans-serif";color:black'><o:p> </o:p></span></p><div><div style='border:none;border-top:solid #B5C4DF 1.0pt;padding:3.0pt 0cm 0cm 0cm'><p class=MsoNormal><b><span lang=EN-US style='font-size:10.0pt;font-family:"Tahoma","sans-serif"'>From:</span></b><span lang=EN-US style='font-size:10.0pt;font-family:"Tahoma","sans-serif"'> rasmb-bounces@list.rasmb.org [mailto:rasmb-bounces@list.rasmb.org] <b>On Behalf Of </b>Sabine Kaltofen<br><b>Sent:</b> 19 August 2013 13:02<br><b>To:</b> HGSR (Holger Martin Strauss); rasmb@list.rasmb.org<br><b>Subject:</b> Re: [RASMB] capillary viscosimeter<o:p></o:p></span></p></div></div><p class=MsoNormal><o:p> </o:p></p><div><p class=MsoNormal style='margin-bottom:12.0pt'><span style='font-size:10.0pt;font-family:"Tahoma","sans-serif";color:black'>Hi Holger and all,<br><br>have you thought of coating the capillary with a silanizing agant such as SigmaCote that is used in crystallography? It works fine for quartz cuvettes and forms a fairly stable film (you have to boil it in detergent to remove it). I guess you have to recalibrate your capillary, though.<br><br>Best<br>Sabine<o:p></o:p></span></p><div><p class=MsoNormal><span style='font-size:10.0pt;font-family:"Tahoma","sans-serif";color:black'><o:p> </o:p></span></p><div><div><p class=MsoNormal><span style='font-size:10.0pt;font-family:"Arial","sans-serif";color:black'>Mind your carbon footprint: please don't print this message.<br></span><span style='font-size:10.0pt;font-family:"Tahoma","sans-serif";color:black'><br><br></span><span style='font-size:10.0pt;font-family:"Arial","sans-serif";color:black'>Sabine Kaltofen<br><br></span><span class=apple-style-span><span style='font-family:"Arial","sans-serif"'><o:p></o:p></span></span></p><div><p class=MsoNormal><span class=apple-style-span><span style='font-size:10.0pt;font-family:"Arial","sans-serif";color:black'>Biochemistry & Structural Biology</span></span><o:p></o:p></p></div><div><p class=MsoNormal><span class=apple-style-span><span style='font-size:10.0pt;font-family:"Arial","sans-serif";color:black'>Lund University</span></span><span style='font-size:10.0pt;font-family:"Arial","sans-serif";color:black'><br><span class=apple-style-span>+46 462220851</span><br><a href="mailto:sabine.kaltofen@biochemistry.lu.se">sabine.kaltofen@biochemistry.lu.se</a><o:p></o:p></span></p></div></div></div></div><div><div class=MsoNormal align=center style='text-align:center'><span style='font-size:12.0pt;font-family:"Times New Roman","serif";color:black'><hr size=2 width="100%" align=center></span></div><div id=divRpF920653><p class=MsoNormal style='margin-bottom:12.0pt'><b><span style='font-size:10.0pt;font-family:"Tahoma","sans-serif";color:black'>From:</span></b><span style='font-size:10.0pt;font-family:"Tahoma","sans-serif";color:black'> rasmb-bounces@list.rasmb.org [rasmb-bounces@list.rasmb.org] on behalf of HGSR (Holger Martin Strauss) [hgsr@novonordisk.com]<br><b>Sent:</b> 19 August 2013 10:46<br><b>To:</b> rasmb@list.rasmb.org<br><b>Subject:</b> [RASMB] capillary viscosimeter</span><span style='font-size:12.0pt;font-family:"Times New Roman","serif";color:black'><o:p></o:p></span></p></div><div><div><p class=MsoNormal><span style='font-size:10.0pt;font-family:"Verdana","sans-serif";color:black'>Hello everyone,</span><span style='color:black'><o:p></o:p></span></p><p class=MsoNormal><span style='font-size:10.0pt;font-family:"Verdana","sans-serif";color:black'> </span><span style='color:black'><o:p></o:p></span></p><p class=MsoNormal><span style='font-size:10.0pt;font-family:"Verdana","sans-serif";color:black'>We’ve recently ventured into measuring viscosities. We use a capillary viscosimeter Lovis 2000 ME from Anton Paar and have observed several times that high concentration protein solutions stick to the (glass) capillary, after rinsing and scrubbing. We then treat the capillary with piranha solution. This is annoying and cumbersome and we haven’t yet found a way to avoid/reduce of protein sticking to the surface. But maybe someone of you has? </span><span style='color:black'><o:p></o:p></span></p><p class=MsoNormal><span style='font-size:10.0pt;font-family:"Verdana","sans-serif";color:black'> </span><span style='color:black'><o:p></o:p></span></p><p class=MsoNormal><span style='font-size:10.0pt;font-family:"Verdana","sans-serif";color:black'>Best wishes, Holger</span><span style='color:black'><o:p></o:p></span></p><p class=MsoNormal><span style='font-size:10.0pt;font-family:"Verdana","sans-serif";color:#3F31F7'> </span><span style='color:black'><o:p></o:p></span></p><p class=MsoNormal><span style='font-size:7.5pt;font-family:"Verdana","sans-serif";color:silver'>_______________________________<br><br></span><b><span style='font-size:7.5pt;font-family:"Verdana","sans-serif";color:black'>Holger Martin Strauss, PhD.</span></b><span style='font-size:7.5pt;font-family:"Verdana","sans-serif";color:black'><br>Senior Scientist<br>Biophysics<br><br>Novo Nordisk A/S<br>Novo Nordisk Park<br>DK-2760 Måløv<br>Denmark<br>+45 4444 8888 (phone)<br>+45 30 75 66 94 (mobile)<br><a href="mailto:hgsr@novonordisk.com">hgsr@novonordisk.com</a><br><br></span><b><span style='font-size:7.5pt;font-family:"Verdana","sans-serif";color:#00CCFF'>Changing</span></b><b><span style='font-size:7.5pt;font-family:"Verdana","sans-serif";color:blue'> diabetes since 1923</span></b><span style='font-size:7.5pt;font-family:"Verdana","sans-serif";color:blue'> <br>Did you know that Novo Nordisk was the first company to introduce insulin to Scandinavia? <u><a href="http://www.novonordisk.com/about_us/changing-diabetes-activities/NN-history.asp" target="_blank">Find out more here</a></u><br><br></span><span style='font-size:7.5pt;font-family:"Verdana","sans-serif";color:black'>This e-mail (including any attachments) is intended for the addressee(s) stated above only and may contain confidential information protected by law. You are hereby notified that any unauthorized reading, disclosure, copying or distribution of this e-mail or use of information contained herein is strictly prohibited and may violate rights to proprietary information. If you are not an intended recipient, please return this e-mail to the sender and delete it immediately hereafter. Thank you.</span><span style='font-size:7.5pt;font-family:"Verdana","sans-serif";color:blue'> </span><span style='color:black'><o:p></o:p></span></p><p class=MsoNormal><span style='font-size:10.0pt;font-family:"Verdana","sans-serif";color:#3F31F7'> </span><span style='color:black'><o:p></o:p></span></p></div></div></div></div></div>
<br><p>This message and any attachment are intended solely for the addressee and may contain confidential information. If you have received this message in error, please send it back to me, and immediately delete it. Please do not use, copy or disclose the information contained in this message or in any attachment. Any views or opinions expressed by the author of this email do not necessarily reflect the views of the University of Nottingham.</p><p>This message has been checked for viruses but the contents of an attachment may still contain software viruses which could damage your computer system, you are advised to perform your own checks. Email communications with the University of Nottingham may be monitored as permitted by UK legislation.</p>
<br></body></html>