[RASMB] capillary viscosimeter

[Sabine Kaltofen]

Hi Holger and all,

have you thought of coating the capillary with a silanizing agant such as SigmaCote that is used in crystallography? It works fine for quartz cuvettes and forms a fairly stable film (you have to boil it in detergent to remove it). I guess you have to recalibrate your capillary, though.

Best
Sabine


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Sabine Kaltofen
Biochemistry & Structural Biology
Lund University
+46 462220851
sabine.kaltofen at biochemistry.lu.se
________________________________
From: rasmb-bounces at list.rasmb.org [rasmb-bounces at list.rasmb.org] on behalf of HGSR (Holger Martin Strauss) [hgsr at novonordisk.com]
Sent: 19 August 2013 10:46
To: rasmb at list.rasmb.org
Subject: [RASMB] capillary viscosimeter

Hello everyone,

We’ve recently ventured into measuring viscosities. We use a capillary viscosimeter Lovis 2000 ME from Anton Paar and have observed several times that high concentration protein solutions stick to the (glass) capillary, after rinsing and scrubbing. We then treat the capillary with piranha solution. This is annoying and cumbersome and we haven’t yet found a way to avoid/reduce of protein sticking to the surface. But maybe someone of you has?

Best wishes, Holger

_______________________________

Holger Martin Strauss, PhD.
Senior Scientist
Biophysics

Novo Nordisk A/S
Novo Nordisk Park
DK-2760 Måløv
Denmark
+45 4444 8888 (phone)
­+45 30 75 66 94 (mobile)
hgsr at novonordisk.com

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