[RASMB] torque stands and isodesmic models

[Roy Hantgan]

Good day, Y'All:

 

I have 2 questions, one practical, the other a bit more abstract:

 

1.	The practical: Any advice on repairing a broken torque stand?
The handle on ours spins and spins w/o tightening up on the collett that
holds the AU cell. We took it apart to find what appears to be a set
screw, i.e. a threaded rod with a ball ending, rather worn. Can that the
culprit? If so, what to do? We do know a machinist...

 

2.	The abstract: Isodesmic association model - OK, I got a nice fit
on some data using Heteroanalysis for a system where the monomer is ~ 9K
and Mw ~ 80 K. So now I would like to calculate/estimate the
concentrations of a range of oligomers, using the resultant Ka and the
input protein concentration. I can solve a monomer-dimer equilibrium
algebraically, possibly a monomer-dimer-trimer but surely not a 9th
degree polynomial. Are there numerical methods? Better yet, software
that will do the hard work for me? 

 

Thanks in advance!

Roy 

 

Roy R. Hantgan, PhD

Associate Professor of  Biochemistry 

Associate in Molecular Medicine

Director, Macromolecular Interactions Core Laboratory

 

Wake Forest University School of Medicine

Medical Center Boulevard 

Winston-Salem, NC 27157

336-716-4675

 

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