[RASMB] Re: Red XLA (Re: Am I missing something?)

[Tom Laue]

Hi all-
A Xe arc lamp serves as the continuous source for the new system. 
However, it would be possible to use a Xe-Hg lamp in the same housing. 
This will provide more lines in the visible and IR, but will do so at 
the expense of the intensity of the UV lines (due to the Hg vapor). I 
will keep this request in mind, though, as we go forward with the 
commercialization.
Best to everyone-
Tom

Dror Noy wrote:

> Dear Tom,
> I think pseudo-absorbnance is indeed the best solution right now as 
> was  suggested by many others who responded to my question (thank you 
> all!).
> Regarding the new rapid-scan absorbance system, I understand that 
> this  would be based on continuos illumination light source instead of 
> a  flash lamp. A few years ago I was discussing with Jack Aviv the  
> possibility of extending the spectral range of the XLA to the NIR  
> (800-1000 nm). It turned out that the biggest obstacle for doing that  
> (beside money) was the "blue" spectrum of Xenon flash lamps. With 
> your  new light source  perhaps it's worth considering extending the 
> spectral  range to the red as well. I wonder if there's anybody else 
> who would be  interested in such an extension. My motivation is to 
> study light  harvesting complexes from purple photosynthetic bacteria 
> which form  protein-pigment complexes absorbing between 800 to 900 nm.
> Best,
> Dror
>
> On 09/06/2005, at 17:10, Tom Laue wrote:
>
>> Dear Dror-
>> It won't help you right now, but we are in the process of 
>> transferring  our "rapid-scan absorbance" system technology to Aviv 
>> Biomedical for  commercial production. This system requires about 1 
>> minute to acquire  data from ALL of the samples (20 micron radial 
>> increments, 5  revolutions averaged... about 50 -150 intensities per 
>> data point). We  are hoping to have alpha versions available in about 
>> a year- I am,  however, an eternal optimist. A paper describing the 
>> technology was  just accepted. Meanwhile, the tip to use 
>> psuedo-absorbance data will  be helpful.
>> Best wishes,
>> Tom Laue
>>
>> Dror Noy wrote:
>>
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>>> Dear all,
>>> I'm running sedimentation velocity experiments on our XLA where a  
>>> typical scan (no replicates, r=6.0 to 7.2) takes about 1 min. If I  
>>> try to scan two cells or more, the time interval between scans  
>>> increases proportionally because the XLA software scans the cells 
>>> one  at a time (i.e. scans the whole radial range for cell 1 then 
>>> cell 2  etc.). This makes scanning several cells unreasonably slow. 
>>> Does  anybody know if it's possible to scan multiple cells 
>>> simultaneously a  radial step at a time (i.e. measure at r=x for all 
>>> the cells then for  r = x+dx for all cells etc.)?
>>> Thanks,
>>> Dror
>>> =========================
>>> Dror Noy, Ph.D.
>>> Structural Biology Dept.
>>> Weizmann Institute of Science
>>> Rehovot 76100
>>> Israel
>>>
>>> e-mail: dror.noy at weizmann.ac.il
>>> Tel: (972)-8-934 2525
>>> Fax: (972)-8-934 4154
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>>
>> -- 
>> Department of Biochemistry and Molecular Biology
>> University of New Hampshire
>> Durham, NH 03824-3544
>> Phone: 603-862-2459
>> FAX:   603-862-0031
>> E-mail: Tom.Laue at unh.edu
>> www.bitc.unh.edu
>> www.camis.unh.edu
>>
>>
> =========================
> Dror Noy, Ph.D.
> Structural Biology Dept.
> Weizmann Institute of Science
> Rehovot 76100
> Israel
>
> e-mail: dror.noy at weizmann.ac.il
> Tel: (972)-8-934 2525
> Fax: (972)-8-934 4154
>

-- 
Department of Biochemistry and Molecular Biology
University of New Hampshire
Durham, NH 03824-3544
Phone: 603-862-2459
FAX:   603-862-0031
E-mail: Tom.Laue at unh.edu
www.bitc.unh.edu
www.camis.unh.edu