[RASMB] Les Holladay

[John Philo]

Yes, Les was an important advocate for AUC and a pioneer who will be missed.

I think very few people in the AUC community know that Les was actually the first person to do direct fitting of multiple sedimentation velocity scans to Lamm equation solutions (an approximate analytical solution that Les developed himself, in this case), all the way back in 1980. One reason this isn’t widely known is that he was so far ahead on this that the term “global analysis" had not been invented, and thus when you read his 1980 paper [1] it is easy to miss that he was simultaneously fitting the entire set of scans. 

It’s also important to remember that after leaving Beckman he was one of the very first people to bring AUC into the pharmaceutical industry. Lastly I will note that his suite of utility programs could calculate protein dn/dc values at least 2 decades ago. 

John

[1] Holladay, L. A. (1980). Simultaneous rapid estimation of sedimentation coefficient and molecular weight. Biophys. Chem. 11, 303-308.

From: RASMB <rasmb-bounces at list.rasmb.org> On Behalf Of Walter Stafford
Sent: Friday, April 7, 2023 9:55 AM
To: rasmb at rasmb.org
Subject: [RASMB] Les Holladay

 

 

From: Thomas Laue <Tom.Laue at unh.edu <mailto:Tom.Laue at unh.edu> >

Subject: Les Holladay

Date: April 7, 2023 at 09:35:06 EDT

To: RASMB <rasmb-bounces at list.rasmb.org <mailto:rasmb-bounces at list.rasmb.org> >

 

Hi all-

Les Holladay passed away at his Tennessee home 3-28-2023 after a bad fall. Many of you will remember Les from his work at Beckman in the 1980s and early 90s on software for the then brand-new XLA ultracentrifuge. His approach to data analysis was thorough and often novel. Within the company he was a champion of the instrument and its capabilities and, at times, felt frustrated that fixes and improvements to the XLA/I weren’t forthcoming (e.g. wavelength setting). Following his departure from Beckman he wrote a series of ‘utility programs’ for solution physical chemistry. These, again, were both useful and often novel.

Best wishes,

Tom

 

 

Professor Emeritus

University of New Hampshire

 

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