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</o:shapelayout></xml><![endif]--></head><body lang=EN-US link=blue vlink=purple style='word-wrap:break-word'><div class=WordSection1><p class=MsoNormal style='margin-bottom:6.0pt'>Yes, Les was an important advocate for AUC and a pioneer who will be missed.<o:p></o:p></p><p class=MsoNormal style='margin-bottom:6.0pt'>I think very few people in the AUC community know that Les was actually the first person to do direct fitting of multiple sedimentation velocity scans to Lamm equation solutions (an approximate analytical solution that Les developed himself, in this case), all the way back in <b>1980</b>. One reason this isn’t widely known is that he was so far ahead on this that the term “global analysis" had not been invented, and thus when you read his 1980 paper [1] it is easy to miss that he was simultaneously fitting the entire set of scans. <o:p></o:p></p><p class=MsoNormal style='margin-bottom:6.0pt'>It’s also important to remember that after leaving Beckman he was one of the very first people to bring AUC into the pharmaceutical industry. Lastly I will note that his suite of utility programs could calculate protein <i>dn/dc</i> values at least 2 decades ago. <o:p></o:p></p><p class=MsoNormal style='margin-bottom:6.0pt'>John<o:p></o:p></p><p class=MsoNormal style='margin-bottom:6.0pt'>[1]<span style='font-size:10.0pt;font-family:"Arial",sans-serif'> </span>Holladay, L. A. (1980). Simultaneous rapid estimation of sedimentation coefficient and molecular weight. <i>Biophys. Chem. </i>11, 303-308.<o:p></o:p></p><div><div style='border:none;border-top:solid #E1E1E1 1.0pt;padding:3.0pt 0in 0in 0in'><p class=MsoNormal><b>From:</b> RASMB <rasmb-bounces@list.rasmb.org> <b>On Behalf Of </b>Walter Stafford<br><b>Sent:</b> Friday, April 7, 2023 9:55 AM<br><b>To:</b> rasmb@rasmb.org<br><b>Subject:</b> [RASMB] Les Holladay<o:p></o:p></p></div></div><p class=MsoNormal><o:p> </o:p></p><div><p class=MsoNormal><o:p> </o:p></p></div><div><p class=MsoNormal><b><span style='font-family:"Helvetica",sans-serif;color:#7F7F7F'>From: </span></b><span style='font-family:"Helvetica",sans-serif'>Thomas Laue <<a href="mailto:Tom.Laue@unh.edu">Tom.Laue@unh.edu</a>></span><span style='font-family:"Times-Roman",serif'><o:p></o:p></span></p></div><div><p class=MsoNormal><b><span style='font-family:"Helvetica",sans-serif;color:#7F7F7F'>Subject: </span></b><b><span style='font-family:"Helvetica",sans-serif'>Les Holladay</span></b><span style='font-family:"Times-Roman",serif'><o:p></o:p></span></p></div><div><p class=MsoNormal><b><span style='font-family:"Helvetica",sans-serif;color:#7F7F7F'>Date: </span></b><span style='font-family:"Helvetica",sans-serif'>April 7, 2023 at 09:35:06 EDT</span><span style='font-family:"Times-Roman",serif'><o:p></o:p></span></p></div><div><p class=MsoNormal><b><span style='font-family:"Helvetica",sans-serif;color:#7F7F7F'>To: </span></b><span style='font-family:"Helvetica",sans-serif'>RASMB <<a href="mailto:rasmb-bounces@list.rasmb.org">rasmb-bounces@list.rasmb.org</a>></span><span style='font-family:"Times-Roman",serif'><o:p></o:p></span></p></div><p class=MsoNormal style='margin-bottom:12.0pt'><o:p> </o:p></p><div><p class=MsoNormal><span style='font-size:14.0pt;font-family:"Times New Roman",serif'>Hi all-</span><o:p></o:p></p></div><div><p class=MsoNormal><span style='font-size:14.0pt;font-family:"Times New Roman",serif'>Les Holladay passed away at his Tennessee home 3-28-2023 after a bad fall. Many of you will remember Les from his work at Beckman in the 1980s and early 90s on software for the then brand-new XLA ultracentrifuge. His approach to data analysis was thorough and often novel. Within the company he was a champion of the instrument and its capabilities and, at times, felt frustrated that fixes and improvements to the XLA/I weren’t forthcoming (e.g. wavelength setting). Following his departure from Beckman he wrote a series of ‘utility programs’ for solution physical chemistry. These, again, were both useful and often novel.</span><o:p></o:p></p></div><div><p class=MsoNormal><span style='font-size:14.0pt;font-family:"Times New Roman",serif'>Best wishes,</span><o:p></o:p></p></div><div><p class=MsoNormal><span style='font-size:14.0pt;font-family:"Times New Roman",serif'>Tom</span><o:p></o:p></p></div><div><p class=MsoNormal><span style='font-size:14.0pt;font-family:"Times New Roman",serif'> </span><o:p></o:p></p></div><div><p class=MsoNormal><span style='font-size:14.0pt;font-family:"Times New Roman",serif'> </span><o:p></o:p></p></div><div><p class=MsoNormal>Professor Emeritus<o:p></o:p></p></div><div><p class=MsoNormal>University of New Hampshire<o:p></o:p></p></div><div><div><p class=MsoNormal><span style='color:black'><o:p> </o:p></span></p></div></div></div></body></html>