[RASMB] FDS hardware question

[Curth.Ute at mh-hannover.de]

Hi John,
With our machine it happens sometimes that the PMT voltage that is used for measuring the signal of the calibration strip changes to values that low that the fluorescence intensity is below the threshold. This results in the same error message, since the calibration strip cannot be detected.
The PMT voltage settings for the calibration strip you can easily check and change in the 'Fluorescence focusing' menu.
Best wishes,
Ute


--
Prof. Dr. rer. nat. Ute Curth
Hannover Medical School
Institute for Biophysical Chemistry
OE 4350, Carl-Neuberg-Str. 1, 30625 Hannover, Germany
Tel.: +49 511 532-9372
curth.ute at mh-hannover.de
www.mh-hannover.de/bpc.html

Von: RASMB [mailto:rasmb-bounces at list.rasmb.org] Im Auftrag von John Sumida
Gesendet: Mittwoch, 30. Oktober 2019 02:36
An: RASMB
Betreff: [RASMB] FDS hardware question

Dear RASMB,

This is a question regarding the AVIV FDS system which is installed in our Proteome XLI instrument.

The data acquisition board on our XLI failed this past summer and we recently had a new board installed.  The Beckman portion of the instrument appears to be fully functional, however the AVIV AOS software was not able to determine the magnet angle in repeated attempts, returning the error: "Calibration strip outside of size limits."

I've confirmed that the stepper motors and their calibration points are correctly listed in the AOS software as well as in the *.RTF file.

I would be curious to know if anyone else has observed this issue on their FDS systems and if so how was it resolved?

Thanks to all for your comments and time in considering my question.

John Sumida.
Molecular Analysis Facility
University of Washington.

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