[RASMB] Optical alignment for AUC

William F Weiss IV weiss_william_f_iv at lilly.com
Wed May 24 05:38:58 PDT 2017


Fellow RASMBers,
If you have ever struggled with alignment problems when using SV-AUC to quantify levels of soluble aggregates, then you might find this interesting:

An optical alignment system improves precision of soluble aggregate quantitation by sedimentation velocity analytical ultracentrifugation
ABSTRACT:
Appropriate characterization of soluble aggregates is an important aspect of biologics development and manufacturing, and sedimentation velocity analytical ultracentrifugation (SV-AUC) is often used an orthogonal technique to size-exclusion chromatography (SEC) for this purpose. Precise quantification of low levels of soluble aggregates by SV-AUC can be adversely impacted by improper cell alignment. This report describes the development of an optical system capable of quantifying cell alignment that affords a substantial improvement compared to historical approaches.

The journal provides temporary free access to the article:

https://authors.elsevier.com/a/1V5iolbALfkA

We look forward to future developments in the field. Thanks.
-Will


William F. Weiss IV, Ph.D.
Principal Research Scientist
Biophysical Characterization Group Leader
Biopharmaceutical Research and Development
Eli Lilly and Company
317.433.8260 (office) | 317.209.4748 (mobile)
weisswf at lilly.com<mailto:weisswf at lilly.com> | www.lilly.com<http://www.lilly.com>

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