[RASMB] Fluorophore with Interference Optics

[Chad Brautigam]

Hi, Robert,

We have used Alexa488-labeled proteins with no problems manifesting in the interference optics.  Since FITC is related to Alexa488, I’m guessing you’ll be OK.  If you’re looking to quantify the amount of bound ligand in a multisignal experiment, that might be problematic, because the ligand’s extinction coefficient could be significantly affected by binding to your protein.

Chad

From: RASMB [mailto:rasmb-bounces at list.rasmb.org] On Behalf Of Robert Shaffer
Sent: Tuesday, June 02, 2015 3:22 PM
To: rasmb at list.rasmb.org
Subject: [RASMB] Fluorophore with Interference Optics

If I use a FITC-labeled ligand with my protein, will the fluorophore cause any issue with the interference optics for a sedimentation velocity run I plan to do next week? Or, does fluorescein not affect the quality of interference data?

Thank you,
Rob

--
Robert Shaffer
rshaff at bu.edu<mailto:rshaff at bu.edu>
MCBB, PhD Program
Boston University


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