[RASMB] Fluorophore with Interference Optics

[Karl Maluf]

Rob,
 
Regarding the actual RI signal (as was already pointed out), the RI
detection system measures the difference in the speed of light between the
reference and sample chambers.  Even if the RI laser will excite your
fluorophore (I'm pretty sure the wavelength is too long to excite
fluorescein), I can't see how there could possibly be enough fluoresence to
compete with the intensity of light going through the sample chamber from
the laser to cause any kind of corrupting fringe displacement.
 
The label certainly can affect the dn/dc of the conjugated protein, but it
is probably quite small (it's a weight average calculation). In this case it
seems the issue is whether or not the fluorphore affects your protein, not
the analytics.
 
Regards,
 
Karl
******************************

N. Karl Maluf, Ph.D.
Senior Research Scientist
Alliance Protein Laboratories, Inc.
6042 Cornerstone Court West, Suite A
San Diego, CA 92121 U.S.A

PH: 1-858-550-9401
Fax: 1-858-550-9403 


  _____  

From: RASMB [mailto:rasmb-bounces at list.rasmb.org] On Behalf Of Robert
Shaffer
Sent: Tuesday, June 02, 2015 1:22 PM
To: rasmb at list.rasmb.org
Subject: [RASMB] Fluorophore with Interference Optics


If I use a FITC-labeled ligand with my protein, will the fluorophore cause
any issue with the interference optics for a sedimentation velocity run I
plan to do next week? Or, does fluorescein not affect the quality of
interference data? 

Thank you,
Rob


-- 

Robert Shaffer
rshaff at bu.edu
MCBB, PhD Program
Boston University


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