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<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D">Hi, Robert,<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D">We have used Alexa488-labeled proteins with no problems manifesting in the interference optics. Since FITC is related to Alexa488, I’m guessing you’ll be OK.
If you’re looking to quantify the amount of bound ligand in a multisignal experiment, that might be problematic, because the ligand’s extinction coefficient could be significantly affected by binding to your protein.<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D">Chad<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D"><o:p> </o:p></span></p>
<p class="MsoNormal"><b><span style="font-size:11.0pt;font-family:"Calibri",sans-serif">From:</span></b><span style="font-size:11.0pt;font-family:"Calibri",sans-serif"> RASMB [mailto:rasmb-bounces@list.rasmb.org]
<b>On Behalf Of </b>Robert Shaffer<br>
<b>Sent:</b> Tuesday, June 02, 2015 3:22 PM<br>
<b>To:</b> rasmb@list.rasmb.org<br>
<b>Subject:</b> [RASMB] Fluorophore with Interference Optics<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:9.5pt">If I use a FITC-labeled ligand with my protein, will the fluorophore cause any issue with the interference optics</span> for a sedimentation velocity run I plan to do next week? Or, does fluorescein not affect
the quality of interference data?<o:p></o:p></p>
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<p class="MsoNormal">Thank you,<o:p></o:p></p>
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<p class="MsoNormal">Rob<br clear="all">
<o:p></o:p></p>
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<p class="MsoNormal">-- <o:p></o:p></p>
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<p class="MsoNormal">Robert Shaffer<o:p></o:p></p>
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<p class="MsoNormal"><a href="mailto:rshaff@bu.edu">rshaff@bu.edu</a><br>
MCBB, PhD Program<br>
Boston University<o:p></o:p></p>
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