[RASMB] Amyloid fibrils and Fujita approach

[Luitgard Nagel-Steger]

Dear Sabine,

reading your question a paper from Geoff Howlett's group came to my 
mind, where they modeled Apolipoprotein C II fibrils with bead models of 
wormlike chains:
> Biophys J. 2003 April; 84(4): 2562–2569.
> PMCID: PMC1302822
> Sedimentation Velocity Analysis of Flexible Macromolecules: Self-Association and Tangling of Amyloid Fibrils
> Christopher A. MacRaild,* Danny M. Hatters,* Lynne J. Lawrence,† and Geoffrey J. Howlett*

A general transformation of all s-value species to MW species might be 
sometimes problematic due to the often heterogeneous nature of 
aggregates present in solutions of amyloidogenic proteins.

Best regards,

Luitgard


Am 04.04.2013 20:34, schrieb Sabine Kaltofen:
>
>
> Dear all,
>
> has anyone of you tried to describe the size/molecular weight of amyloid
> fibrils using the Fujita approach?
> I am after a rather accurate determination of the MW (distribution) of
> fibrils.
> Therefore, using a scaling law to relate s with M seems the most
> rigorous thing to do. I am, however not aware of any literature where
> this approach has been used for peptide/protein fibrils.
> Am I missing a major point here, why this is not applicable?
>
> All comments, experiences and thoughts are greatly appreciated!
>
> Best wishes
>
> Sabine
>
>
>
> Mind your carbon footprint: please don't print this message.
>
>
> Sabine Kaltofen
> Biochemistry & Structural Biology
> Lund University
> sabine.kaltofen at biochemistry.lu.se
>
>
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