[RASMB] Launch of SEDFIT-MSTAR for sed. eqm analysis of polymers

[Stephen Harding]

Dear all

With Peter Schuck, Richard Gillis, David Besong, Fahad Almutairi, Gary Adams and Arthur Rowe we are happy to announce the launch of the algorithm SEDFIT-MSTAR for the analysis of sedimentation equilibrium data of solutions of polymers.

The SEDFIT-MSTAR procedure - which takes only a few minutes to perform - uses a "smart smooth" principle for baseline determination for interference and absorption optical data (including estimation of the concentration at the air/solution meniscus) and provides

(i)           an accurate estimate for the (apparent) weight average molecular weight for the whole distribution of species in the sample

(ii)          the variation in "point" average molecular weight with both radius and local concentration in the ultracentrifuge cell

(iii)         an estimate for the molecular weight distribution

The method has just been published in the Analyst:

SCHUCK P, GILLIS RB, BESONG D, ALMUTAIRI F, ADAMS GG, ROWE AJ and HARDING SE, 2014. SEDFIT-MSTAR: Molecular weight and molecular weight distribution analysis of polymers by sedimentation equilibrium in the ultracentrifuge<http://pubs.rsc.org/en/content/articlepdf/2013/an/c3an01507f?page=search> Analyst, 139, 79-92

and can be accessed/downloaded from the Royal Society of Chemistry's website on

http://pubs.rsc.org/en/content/articlepdf/2013/an/c3an01507f?page=search

{let us know if you have difficulty accessing this article}



The programme (SEDFIT-MSTARv1 and associated files) can be downloaded from the NIH's SEDFIT page:

https://sedfitsedphat.nibib.nih.gov/software/Shared%20Documents/SEDFIT-MSTAR.zip

There is a link to this also from the NCMH software page:

http://www.nottingham.ac.uk/ncmh/unit/method.html#Software





The download includes

1.    SEDFIT-MSTARv1

2.    SEDFIT MSTAR manual (a series of powerpoint screens which takes you through the analysis)

3.    4 datasets based on simulated data - all illustrated in the Analyst paper (ideal single solute, ideal 2-solute, ideal 2-solute with data error, a highly non-ideal data-set (equivalent to a protein concentration ~ 20 mg/ml)

4.    3 data sets for real systems - also illustrated in the Analyst paper: IgG1, pullulan and lambda-carrageenan



SEDFIT-MSTAR complements the new "Extended Fujita" algorithm - also available on the SEDFIT platform for the analysis of polymer molecular weight distributions using sedimentation velocity - and SEDPHAT for the more detailed sedimentation equilibrium analysis of protein based systems (including self-association and interaction analysis); and MULTISIG for the more detailed component analysis of polydisperse systems.

Let us know if you have any difficulties in running the programme and we will do our best to help!



Seasons Greetings,



Steve (& Peter, Rich, David, Fahad, Gary and Arthur)





**********************************************************

Stephen E. Harding www.nottingham.ac.uk/Biosciences/People/steve.harding<http://www.nottingham.ac.uk/Biosciences/People/steve.harding>

Ridder den Kongelige Norske Fortjenstorden,

Professor of Applied Biochemistry, University of Nottingham

E-post: steve.harding at nottingham.ac.uk<mailto:steve.harding at nottingham.ac.uk>





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