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</o:shapelayout></xml><![endif]--></head><body lang=EN-GB link=blue vlink=purple><div class=WordSection1><p class=MsoPlainText>Dear all<o:p></o:p></p><p class=MsoPlainText>With Peter Schuck, Richard Gillis, David Besong, Fahad Almutairi, Gary Adams and Arthur Rowe we are happy to announce the launch of the algorithm SEDFIT-MSTAR for the analysis of sedimentation equilibrium data of solutions of polymers.<o:p></o:p></p><p class=MsoPlainText>The SEDFIT-MSTAR procedure – which takes only a few minutes to perform – uses a “smart smooth” principle for baseline determination for interference and absorption optical data (including estimation of the concentration at the air/solution meniscus) and provides<o:p></o:p></p><p class=MsoPlainText style='margin-left:54.0pt;text-indent:-36.0pt;mso-list:l1 level1 lfo2'><![if !supportLists]><span style='mso-list:Ignore'>(i)<span style='font:7.0pt "Times New Roman"'> </span></span><![endif]>an accurate estimate for the (apparent) weight average molecular weight for the whole distribution of species in the sample<o:p></o:p></p><p class=MsoPlainText style='margin-left:54.0pt;text-indent:-36.0pt;mso-list:l1 level1 lfo2'><![if !supportLists]><span style='mso-list:Ignore'>(ii)<span style='font:7.0pt "Times New Roman"'> </span></span><![endif]>the variation in “point” average molecular weight with both radius and local concentration in the ultracentrifuge cell<o:p></o:p></p><p class=MsoPlainText style='margin-left:54.0pt;text-indent:-36.0pt;mso-list:l1 level1 lfo2'><![if !supportLists]><span style='mso-list:Ignore'>(iii)<span style='font:7.0pt "Times New Roman"'> </span></span><![endif]>an estimate for the molecular weight distribution<o:p></o:p></p><p class=MsoPlainText>The method has just been published in the <i>Analyst</i>: <o:p></o:p></p><p class=MsoPlainText><span class=citationarticleauthors><span lang=EN style='font-size:9.0pt;color:#535353'>SCHUCK P, GILLIS RB, BESONG D, ALMUTAIRI F, ADAMS GG, ROWE AJ and HARDING SE, </span></span><span class=citationarticleyear><span lang=EN style='font-size:9.0pt;color:#535353'>2014. </span></span><span class=citationarticletitle><span lang=EN style='font-size:9.0pt;color:#535353'><a href="http://pubs.rsc.org/en/content/articlepdf/2013/an/c3an01507f?page=search"><span style='color:#535353'>SEDFIT-MSTAR: Molecular weight and molecular weight distribution analysis of polymers by sedimentation equilibrium in the ultracentrifuge</span></a> </span></span><span class=citationjournaltitle><i><span lang=EN style='font-size:9.0pt;color:#535353'>Analyst</span></i></span><span class=citationjournaltitle><span lang=EN style='font-size:9.0pt;color:#535353'>, </span></span><span class=citationjournalvolumeandpart><b><span lang=EN style='font-size:9.0pt;color:#535353'>139</span></b></span><span class=citationjournalvolumeandpart><span lang=EN style='font-size:9.0pt;color:#535353'>, </span></span><span class=citationjournalpages><span lang=EN style='font-size:9.0pt;color:#535353'>79-92<o:p></o:p></span></span></p><p class=MsoPlainText><span class=citationjournalpages><span lang=EN style='font-size:9.0pt;color:#535353'>and can be accessed/downloaded from the Royal Society of Chemistry’s website on<o:p></o:p></span></span></p><p class=MsoPlainText><span class=citationjournalpages><span lang=EN style='font-size:9.0pt;color:#535353'><a href="http://pubs.rsc.org/en/content/articlepdf/2013/an/c3an01507f?page=search">http://pubs.rsc.org/en/content/articlepdf/2013/an/c3an01507f?page=search</a><o:p></o:p></span></span></p><p class=MsoPlainText><span class=citationjournalpages><span lang=EN style='font-size:9.0pt;color:#535353'>{let us know if you have difficulty accessing this article}<o:p></o:p></span></span></p><p class=MsoPlainText><span class=citationjournalpages><span lang=EN style='font-size:9.0pt;color:#535353'><o:p> </o:p></span></span></p><p class=MsoPlainText><span class=citationjournalpages><span lang=EN>The programme (SEDFIT-MSTARv1 and associated files) can be downloaded from the NIH’s SEDFIT page:<o:p></o:p></span></span></p><p class=MsoPlainText><a href="https://sedfitsedphat.nibib.nih.gov/software/Shared%20Documents/SEDFIT-MSTAR.zip">https://sedfitsedphat.nibib.nih.gov/software/Shared%20Documents/SEDFIT-MSTAR.zip</a><o:p></o:p></p><p class=MsoPlainText><span class=citationjournalpages><span lang=EN style='font-size:9.0pt;color:#535353'>There is a link to this also from the NCMH software page:<o:p></o:p></span></span></p><p class=MsoPlainText><span class=citationjournalpages><span lang=EN style='font-size:9.0pt;color:#535353'><a href="http://www.nottingham.ac.uk/ncmh/unit/method.html#Software">http://www.nottingham.ac.uk/ncmh/unit/method.html#Software</a><o:p></o:p></span></span></p><p class=MsoPlainText><span class=citationjournalpages><span lang=EN style='font-size:9.0pt;color:#535353'><o:p> </o:p></span></span></p><p class=MsoPlainText><span class=citationjournalpages><span lang=EN style='font-size:9.0pt;color:#535353'><o:p> </o:p></span></span></p><p class=MsoPlainText><span class=citationjournalpages><span lang=EN style='font-size:9.0pt;color:#535353'>The download includes<o:p></o:p></span></span></p><p class=MsoPlainText style='margin-left:36.0pt;text-indent:-18.0pt;mso-list:l0 level1 lfo4'><![if !supportLists]><span class=citationjournalpages><span lang=EN style='font-size:9.0pt;color:#535353'><span style='mso-list:Ignore'>1.<span style='font:7.0pt "Times New Roman"'> </span></span></span></span><![endif]><span class=citationjournalpages><span lang=EN style='font-size:9.0pt;color:#535353'>SEDFIT-MSTARv1 <o:p></o:p></span></span></p><p class=MsoPlainText style='margin-left:36.0pt;text-indent:-18.0pt;mso-list:l0 level1 lfo4'><![if !supportLists]><span class=citationjournalpages><span lang=EN style='font-size:9.0pt;color:#535353'><span style='mso-list:Ignore'>2.<span style='font:7.0pt "Times New Roman"'> </span></span></span></span><![endif]><span class=citationjournalpages><span lang=EN style='font-size:9.0pt;color:#535353'>SEDFIT MSTAR manual (a series of powerpoint screens which takes you through the analysis)<o:p></o:p></span></span></p><p class=MsoPlainText style='margin-left:36.0pt;text-indent:-18.0pt;mso-list:l0 level1 lfo4'><![if !supportLists]><span class=citationjournalpages><span lang=EN style='font-size:9.0pt;color:#535353'><span style='mso-list:Ignore'>3.<span style='font:7.0pt "Times New Roman"'> </span></span></span></span><![endif]><span class=citationjournalpages><span lang=EN style='font-size:9.0pt;color:#535353'>4 datasets based on simulated data – all illustrated in the Analyst paper (ideal single solute, ideal 2-solute, ideal 2-solute with data error, a highly non-ideal data-set (equivalent to a protein concentration ~ 20 mg/ml)<o:p></o:p></span></span></p><p class=MsoPlainText style='margin-left:36.0pt;text-indent:-18.0pt;mso-list:l0 level1 lfo4'><![if !supportLists]><span class=citationjournalpages><span lang=EN style='font-size:9.0pt;color:#535353'><span style='mso-list:Ignore'>4.<span style='font:7.0pt "Times New Roman"'> </span></span></span></span><![endif]><span class=citationjournalpages><span lang=EN style='font-size:9.0pt;color:#535353'>3 data sets for real systems – also illustrated in the Analyst paper: IgG1, pullulan and lambda-carrageenan<o:p></o:p></span></span></p><p class=MsoPlainText><span class=citationjournalpages><span lang=EN style='font-size:9.0pt;color:#535353'><o:p> </o:p></span></span></p><p class=MsoPlainText><span class=citationjournalpages><span lang=EN>SEDFIT-MSTAR complements the new “Extended Fujita” algorithm - also available on the SEDFIT platform for the analysis of polymer molecular weight distributions using sedimentation velocity - and SEDPHAT for the more detailed sedimentation equilibrium analysis of protein based systems (including self-association and interaction analysis); and MULTISIG for the more detailed component analysis of polydisperse systems. <o:p></o:p></span></span></p><p class=MsoPlainText>Let us know if you have any difficulties in running the programme and we will do our best to help! <o:p></o:p></p><p class=MsoPlainText><o:p> </o:p></p><p class=MsoPlainText><span style='color:black'>Seasons Greetings,<o:p></o:p></span></p><p class=MsoPlainText><span style='color:black'><o:p> </o:p></span></p><p class=MsoPlainText>Steve (& Peter, Rich, David, Fahad, Gary and Arthur) <o:p></o:p></p><p class=MsoPlainText> <o:p></o:p></p><p class=MsoPlainText><o:p> </o:p></p><p class=MsoPlainText>**********************************************************<o:p></o:p></p><p class=MsoPlainText>Stephen E. Harding <a href="http://www.nottingham.ac.uk/Biosciences/People/steve.harding">www.nottingham.ac.uk/Biosciences/People/steve.harding</a><o:p></o:p></p><p class=MsoPlainText>Ridder den Kongelige Norske Fortjenstorden,<o:p></o:p></p><p class=MsoPlainText>Professor of Applied Biochemistry, University of Nottingham<o:p></o:p></p><p class=MsoPlainText>E-post: <a href="mailto:steve.harding@nottingham.ac.uk">steve.harding@nottingham.ac.uk</a><o:p></o:p></p><p class=MsoPlainText><o:p> </o:p></p><p class=MsoPlainText><o:p> </o:p></p></div>
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