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<div style="direction: ltr;font-family: Tahoma;color: #000000;font-size: 10pt;">Hi Holger and all,<br>
<br>
have you thought of coating the capillary with a silanizing agant such as SigmaCote that is used in crystallography? It works fine for quartz cuvettes and forms a fairly stable film (you have to boil it in detergent to remove it). I guess you have to recalibrate
your capillary, though.<br>
<br>
Best<br>
Sabine<br>
<br>
<div><br>
<div style="font-family:Tahoma; font-size:13px">
<div style="font-family:Tahoma; font-size:13px"><font face="Arial" size="2">Mind your carbon footprint: please don't print this message.<br>
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<br>
<font face="Arial">Sabine Kaltofen<br>
<span class="Apple-style-span" style="border-collapse:separate; color:rgb(0,0,0); font-style:normal; font-variant:normal; font-weight:normal; letter-spacing:normal; line-height:normal; orphans:2; text-indent:0px; text-transform:none; white-space:normal; widows:2; word-spacing:0px"><span class="Apple-style-span" style="border-collapse:separate; color:rgb(0,0,0); font-style:normal; font-variant:normal; font-weight:normal; letter-spacing:normal; line-height:normal; orphans:2; text-indent:0px; text-transform:none; white-space:normal; widows:2; word-spacing:0px">
<div><span class="Apple-style-span" style="line-height:13px">Biochemistry & Structural Biology</span></div>
<div><span class="Apple-style-span" style="line-height:13px"><font size="2"><font face="Arial"><span class="Apple-style-span" style="border-collapse:separate; color:rgb(0,0,0); font-style:normal; font-variant:normal; font-weight:normal; letter-spacing:normal; line-height:normal; orphans:2; text-indent:0px; text-transform:none; white-space:normal; widows:2; word-spacing:0px"><span class="Apple-style-span" style="border-collapse:separate; color:rgb(0,0,0); font-style:normal; font-variant:normal; font-weight:normal; letter-spacing:normal; line-height:normal; orphans:2; text-indent:0px; text-transform:none; white-space:normal; widows:2; word-spacing:0px"><span class="Apple-style-span" style="line-height:13px">Lund
University<br>
</span></span></span></font></font>+46 462220851<br>
</span><font face="Arial">sabine.kaltofen@biochemistry.lu.se</font><br>
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<div style="direction: ltr;" id="divRpF920653"><font color="#000000" face="Tahoma" size="2"><b>From:</b> rasmb-bounces@list.rasmb.org [rasmb-bounces@list.rasmb.org] on behalf of HGSR (Holger Martin Strauss) [hgsr@novonordisk.com]<br>
<b>Sent:</b> 19 August 2013 10:46<br>
<b>To:</b> rasmb@list.rasmb.org<br>
<b>Subject:</b> [RASMB] capillary viscosimeter<br>
</font><br>
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<p class="MsoNormal"><span style="font-size:10.0pt; font-family:"Verdana","sans-serif"; color:black">Hello everyone,</span></p>
<p class="MsoNormal"><span style="font-size:10.0pt; font-family:"Verdana","sans-serif"; color:black"> </span></p>
<p class="MsoNormal"><span style="font-size:10.0pt; font-family:"Verdana","sans-serif"; color:black">We’ve recently ventured into measuring viscosities. We use a capillary viscosimeter Lovis 2000 ME from Anton Paar and have observed several times that high
concentration protein solutions stick to the (glass) capillary, after rinsing and scrubbing. We then treat the capillary with piranha solution. This is annoying and cumbersome and we haven’t yet found a way to avoid/reduce of protein sticking to the surface.
But maybe someone of you has? </span></p>
<p class="MsoNormal"><span style="font-size:10.0pt; font-family:"Verdana","sans-serif"; color:black"> </span></p>
<p class="MsoNormal"><span style="font-size:10.0pt; font-family:"Verdana","sans-serif"; color:black">Best wishes, Holger</span></p>
<p class="MsoNormal"><span style="font-size:10.0pt; font-family:"Verdana","sans-serif"; color:#3F31F7"> </span></p>
<p class="MsoNormal"><span style="font-size:7.5pt; font-family:"Verdana","sans-serif"; color:silver">_______________________________<br>
<br>
</span><b><span style="font-size:7.5pt; font-family:"Verdana","sans-serif"; color:black">Holger Martin Strauss, PhD.</span></b><span style="font-size:7.5pt; font-family:"Verdana","sans-serif"; color:black"><br>
Senior Scientist<br>
Biophysics<br>
<br>
Novo Nordisk A/S<br>
Novo Nordisk Park<br>
DK-2760 Måløv<br>
Denmark<br>
+45 4444 8888 (phone)<br>
+45 30 75 66 94 (mobile)<br>
hgsr@novonordisk.com<br>
<br>
</span><b><span style="font-size:7.5pt; font-family:"Verdana","sans-serif"; color:#00CCFF">Changing</span></b><b><span style="font-size:7.5pt; font-family:"Verdana","sans-serif"; color:blue"> diabetes since 1923</span></b><span style="font-size:7.5pt; font-family:"Verdana","sans-serif"; color:blue">
<br>
Did you know that Novo Nordisk was the first company to introduce insulin to Scandinavia?
<u><a href="http://www.novonordisk.com/about_us/changing-diabetes-activities/NN-history.asp" target="_blank">Find out more here</a></u><br>
<br>
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