[RASMB] Peptide sedimentation

[Laue, Thomas]

Hi Marina-
I agree with all of the previous respondents.
One last thing to keep in mind is that vbar for a peptide may differ from that calculated for a protein. In general, the actual vbar is smaller than what is  calculated, leading to an overestimate of M (if you use the calculated value).
I believe there is some discussion of this in Sednterp.
Best wishes,
Tom
________________________________
From: rasmb-bounces at list.rasmb.org [rasmb-bounces at list.rasmb.org] on behalf of Fasolini, Marina [Nervianoms] [Marina.Fasolini at nervianoms.com]
Sent: Tuesday, July 09, 2013 5:51 AM
To: rasmb at rasmb.org
Subject: [RASMB] Peptide sedimentation

Dear All
I need to follow the aggregation of some peptides of about 4 KDa . I would like to estimate the dimension of these aggregates.
Since my experience is mostly in protein sedimentation, I was wondering if you have some tips for me. Which wavelength do you use for absorbance optic? Which is the best concentration to run?
At which speed do you suggest me to run the experiment?
Any help will be appreciated.
Thanks in advance
Marina



MARINA FASOLINI
Structural Chemistry

Nerviano Medical Sciences http://www.nervianoms.com<http://www.nervianoms.com/>
Viale Pasteur 10
20014 Nerviano - Milano
marina.fasolini at nervianoms.com
Tel. +390331581462
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