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<div style="direction: ltr;font-family: Tahoma;color: #000000;font-size: 10pt;">Hi Marina-<br>
I agree with all of the previous respondents.<br>
One last thing to keep in mind is that vbar for a peptide may differ from that calculated for a protein. In general, the actual vbar is smaller than what is calculated, leading to an overestimate of M (if you use the calculated value).<br>
I believe there is some discussion of this in Sednterp.<br>
Best wishes,<br>
Tom<br>
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<div style="direction: ltr;" id="divRpF584349"><font color="#000000" face="Tahoma" size="2"><b>From:</b> rasmb-bounces@list.rasmb.org [rasmb-bounces@list.rasmb.org] on behalf of Fasolini, Marina [Nervianoms] [Marina.Fasolini@nervianoms.com]<br>
<b>Sent:</b> Tuesday, July 09, 2013 5:51 AM<br>
<b>To:</b> rasmb@rasmb.org<br>
<b>Subject:</b> [RASMB] Peptide sedimentation<br>
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<div><font face="Arial" size="2"><span class="207421909-09072013">Dear All</span></font></div>
<div><font face="Arial" size="2"><span class="207421909-09072013">I need to follow the aggregation of some peptides of about 4 KDa . I would like to estimate the dimension of these aggregates.
</span></font></div>
<div><font face="Arial" size="2"><span class="207421909-09072013">Since my experience is mostly in protein sedimentation, I was wondering if you have some tips for me. Which wavelength do you use for absorbance optic? Which is the best concentration to run?</span></font></div>
<div><font face="Arial" size="2"><span class="207421909-09072013">At which speed do you suggest me to run the experiment?</span></font></div>
<div><font face="Arial" size="2"><span class="207421909-09072013">Any help will be appreciated.</span></font></div>
<div><font face="Arial" size="2"><span class="207421909-09072013">Thanks in advance</span></font></div>
<div><font face="Arial" size="2"><span class="207421909-09072013">Marina</span></font></div>
<div><font face="Arial" size="2"><span class="207421909-09072013"></span></font> </div>
<div><font face="Arial" size="2"><span class="207421909-09072013"></span></font> </div>
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<div align="left">MARINA FASOLINI<br>
Structural Chemistry<br>
<br>
Nerviano Medical Sciences <a href="http://www.nervianoms.com/" target="_blank">http://www.nervianoms.com</a><br>
Viale Pasteur 10<br>
20014 Nerviano - Milano<br>
marina.fasolini@nervianoms.com <br>
Tel. +390331581462<br>
Fax. +390331581360<br>
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