[RASMB] Correspondence concentration cesium chloride vs fringes

[Antje Voelkel]

Dear Eric,

I think the dn/dc of the CsCl varies from package to package. We ones 
measured a value of 0.0837 ml/g.

With the equation:

*delta c pro fringe shift J*

delta c [g/ml] = J * lambda*10-7  / dn/dc * a


dn/dc [ml/g]

a [cm - 1.2 for standard AUC cells]

lambda = 675 nm (laser)

you are able to calculate every concentration you want to.

(Investigation of quantum size colloids using the XL-I 
ultracentrifuge;/H. Cölfen 
<http://www.springerlink.com/content/?Author=H.+C%c3%b6lfen>//, //T. 
Pauck <http://www.springerlink.com/content/?Author=T.+Pauck>//und //M. 
Antonietti <http://www.springerlink.com/content/?Author=M.+Antonietti>/)


For the simulation of a gradient, you need activity coefficients. I can 
send you an excel sheet for the calculation of the concentration gradient.

The determination of density distributions and density gradients in 
binary solutions at equilibrium in the ultracentrifuge/Ifft J.B., Voet 
D.H. Vinograd J. J.Phys.Chem 65/1961 1138-1145/

//

Best regards

Antje

/

/


Am 29/11/2011 07:33, schrieb Kusznir, Eric Andre:
>
> Hello RASMB Community
>
> I will be grateful if any of you could tell me the correspondence of 
> the gradient concentration of cesium chloride based on the number of 
> interference fringes.
>
> Thanks in advance.
>
> Best regards
>
> Eric
>
>
>
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-- 
Antje Voelkel
Max Planck Institute of Colloids and Interfaces
Am Muehlenberg 1
D-14476 Potsdam
+49(0)331 567 9528/9531

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