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<p class="MsoNormal" style="margin-bottom:12.0pt"><span
style="mso-ansi-language:EN-GB" lang="EN-GB">Dear Eric,<br>
<br>
I think the dn/dc of the CsCl varies from package to package. We
ones measured
a value of 0.0837 ml/g.<br>
<br>
With the equation:<o:p></o:p></span></p>
<p class="MsoNormal"><b style="mso-bidi-font-weight:normal">delta c
pro fringe
shift J<o:p></o:p></b></p>
<p class="MsoNormal">delta c [g/ml] = J * lambda*10-7 / dn/dc * a</p>
<p class="MsoNormal"><o:p> </o:p><br>
dn/dc [ml/g]</p>
<p class="MsoNormal">a [cm - 1.2 for standard AUC cells]</p>
<p class="MsoNormal">lambda = 675 nm (laser)</p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal"><span style="mso-ansi-language:EN-GB"
lang="EN-GB">you are
able to calculate every concentration you want to.<o:p></o:p></span></p>
<p class="MsoNormal"><span
style="font-size:10.0pt;mso-ansi-language:
EN-GB" lang="EN-GB">(</span><span
style="font-size:10.0pt;font-family:Arial;
mso-ansi-language:EN-GB" lang="EN-GB">Investigation of quantum
size colloids using the XL-I
ultracentrifuge;</span><i style="mso-bidi-font-style:normal"><span
style="font-size:10.0pt"><a
href="http://www.springerlink.com/content/?Author=H.+C%c3%b6lfen"
title="View content where Author is H. Cölfen"><span
style="color:windowtext;
mso-ansi-language:EN-GB;text-decoration:none;text-underline:none"> <span
lang="EN-GB">H. Cölfen</span></span></a></span></i><i
style="mso-bidi-font-style:
normal"><span style="font-size:10.0pt;mso-ansi-language:EN-GB"
lang="EN-GB">, </span></i><i
style="mso-bidi-font-style:normal"><span
style="font-size:10.0pt"><a
href="http://www.springerlink.com/content/?Author=T.+Pauck"
title="View content where Author is T. Pauck"><span
style="color:windowtext;mso-ansi-language:EN-GB;text-decoration:none;
text-underline:none" lang="EN-GB">T. Pauck</span></a></span></i><i
style="mso-bidi-font-style:
normal"><span style="font-size:10.0pt;mso-ansi-language:EN-GB"
lang="EN-GB"> und </span></i><i
style="mso-bidi-font-style:normal"><span
style="font-size:10.0pt"><a
href="http://www.springerlink.com/content/?Author=M.+Antonietti"
title="View content where Author is M. Antonietti"><span
style="color:windowtext;mso-ansi-language:EN-GB;text-decoration:none;
text-underline:none" lang="EN-GB">M. Antonietti</span></a></span></i><span
style="font-size:
10.0pt;color:black;mso-ansi-language:EN-GB"> </span><span
style="font-size:10.0pt;mso-ansi-language:EN-GB" lang="EN-GB">)<br>
</span><span style="mso-ansi-language:EN-GB" lang="EN-GB"><br>
<br>
For the simulation of a gradient, you need activity
coefficients. I can send
you an excel sheet for the calculation of the <u2:shapelayout
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gradient.<o:p></o:p></u4:colorscheme></span></p>
<p class="MsoNormal"><span
style="font-size:10.0pt;font-family:Arial">The
determination of density distributions and density gradients in
binary
solutions at equilibrium in the ultracentrifuge</span><span
style="font-size:
10.0pt"> <i style="mso-bidi-font-style:normal">Ifft J.B., Voet
D.H. Vinograd J.
J.Phys.Chem 65/1961 1138-1145<o:p></o:p></i></span></p>
<p class="MsoNormal"><i style="mso-bidi-font-style:normal"><span
style="font-size:10.0pt"><o:p> </o:p></span></i></p>
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<p class="MsoNormal">Best regards</p>
<p class="MsoNormal">Antje</p>
<p class="MsoNormal"><o:p> </o:p></p>
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Am 29/11/2011 07:33, schrieb Kusznir, Eric Andre:
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<p class="MsoNormal"><a moz-do-not-send="true" name="_GoBack"></a><span
style="font-size:12.0pt;line-height:115%;font-family:"Arial","sans-serif"">Hello
RASMB Community<o:p></o:p></span></p>
<p class="MsoNormal"
style="margin-bottom:0cm;margin-bottom:.0001pt;line-height:normal;background:whitesmoke;vertical-align:top"><span
style="font-size:12.0pt;font-family:"Arial","sans-serif";color:#333333"
lang="EN"><o:p> </o:p></span></p>
<p class="MsoNormal"
style="margin-bottom:0cm;margin-bottom:.0001pt;line-height:normal;background:whitesmoke;vertical-align:top"><span
style="font-size:12.0pt;font-family:"Arial","sans-serif";color:#333333"
lang="EN"><o:p> </o:p></span></p>
<p class="MsoNormal"
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style="font-size:12.0pt;font-family:"Arial","sans-serif";color:#333333"
lang="EN">I will be grateful if any of you could tell me the
correspondence of the gradient concentration of cesium
chloride based on the number of interference fringes.<o:p></o:p></span></p>
<p class="MsoNormal"
style="margin-bottom:0cm;margin-bottom:.0001pt;line-height:normal;background:whitesmoke;vertical-align:top"><span
style="font-size:12.0pt;font-family:"Arial","sans-serif";color:#333333"
lang="EN">Thanks in advance.<o:p></o:p></span></p>
<p class="MsoNormal"
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lang="EN"><o:p> </o:p></span></p>
<p class="MsoNormal"
style="margin-bottom:0cm;margin-bottom:.0001pt;line-height:normal;background:whitesmoke;vertical-align:top"><span
style="font-size:12.0pt;font-family:"Arial","sans-serif";color:#333333"
lang="EN">Best regards<o:p></o:p></span></p>
<p class="MsoNormal"
style="margin-bottom:0cm;margin-bottom:.0001pt;line-height:normal;background:whitesmoke;vertical-align:top"><span
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lang="EN"><o:p> </o:p></span></p>
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style="font-size:12.0pt;font-family:"Arial","sans-serif";color:#333333"
lang="EN">Eric</span><span
style="font-size:10.0pt;font-family:"Arial","sans-serif";color:#888888"><o:p></o:p></span></p>
<p class="MsoNormal"><span
style="font-size:10.0pt;line-height:115%;font-family:"Arial","sans-serif""><o:p> </o:p></span></p>
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<pre wrap="">_______________________________________________
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<br>
<pre class="moz-signature" cols="72">--
Antje Voelkel
Max Planck Institute of Colloids and Interfaces
Am Muehlenberg 1
D-14476 Potsdam
+49(0)331 567 9528/9531</pre>
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