[RASMB] Intensity Scan Help

[John Philo]

Peter,
 
If I am understanding you properly then your optics are working just fine.
That average intensity is good, and typically the intensity varies more than
that across the cell. So why do you believe something is wrong?
 
John

  _____  

From: rasmb-bounces at rasmb.bbri.org [mailto:rasmb-bounces at rasmb.bbri.org] On
Behalf Of Peter Edward Prevelige Jr
Sent: Friday, July 22, 2011 9:52 AM
To: rasmb at rasmb.bbri.org
Subject: [RASMB] Intensity Scan Help



Hi All

 

We are still having problems with our XL-A.  I have run intensity scans
across an empty rotor hole at 280  (counterbalances in pos 2 & 4, scan cell
3). I'm not sure I set it up right  but if I did the intensity averages 6700
but varies from about 6500 to 7500. Apart from much lower noise fluctuations
it appears that the reference and sample land on top of one another at all
points.  What sort of variation should I be seeing with radial distance. 

 

What sort of tests should I be doing to evaluate the optical system?

 

 

Thanks

 

Peter

 

Peter E. Prevelige Jr.

Professor

Dept. of Microbiology, BBRB 416/6

Univ. of Alabama @ Birmingham

845 19th St. South

Birmingham AL. 35294-2170

Phone 205 975-5327

FAX 205 975-5479

prevelig at uab.edu

http://www.microbio.uab.edu/faculty/prevelige/prevelige-p.htm

 

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