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<DIV dir=ltr align=left>
<DIV dir=ltr align=left><SPAN class=798423717-22072011><FONT color=#0000ff
size=2 face=Arial>Peter,</FONT></SPAN></DIV>
<DIV dir=ltr align=left><SPAN class=798423717-22072011><FONT color=#0000ff
size=2 face=Arial></FONT></SPAN> </DIV>
<DIV dir=ltr align=left><SPAN class=798423717-22072011><FONT color=#0000ff
size=2 face=Arial>If I am understanding you properly then your optics are
working just fine. That average intensity is good, and typically the intensity
varies more than that across the cell. So why do you believe something is
wrong?</FONT></SPAN></DIV>
<DIV dir=ltr align=left><SPAN class=798423717-22072011><FONT color=#0000ff
size=2 face=Arial></FONT></SPAN> </DIV>
<DIV dir=ltr align=left><SPAN class=798423717-22072011><FONT color=#0000ff
size=2 face=Arial>John</FONT></SPAN></DIV><BR>
<HR tabIndex=-1>
<FONT size=2 face=Tahoma><B>From:</B> rasmb-bounces@rasmb.bbri.org
[mailto:rasmb-bounces@rasmb.bbri.org] <B>On Behalf Of </B>Peter Edward Prevelige
Jr<BR><B>Sent:</B> Friday, July 22, 2011 9:52 AM<BR><B>To:</B>
rasmb@rasmb.bbri.org<BR><B>Subject:</B> [RASMB] Intensity Scan
Help<BR></FONT><BR></DIV>
<DIV></DIV>
<DIV class=WordSection1>
<P class=MsoNormal>Hi All<o:p></o:p></P>
<P class=MsoNormal><o:p> </o:p></P>
<P class=MsoNormal>We are still having problems with our XL-A. I have run
intensity scans across an empty rotor hole at 280 (counterbalances in pos
2 & 4, scan cell 3). I’m not sure I set it up right but if I did the
intensity averages 6700 but varies from about 6500 to 7500. Apart from much
lower noise fluctuations it appears that the reference and sample land on top of
one another at all points. What sort of variation should I be seeing with
radial distance. <o:p></o:p></P>
<P class=MsoNormal><o:p> </o:p></P>
<P class=MsoNormal>What sort of tests should I be doing to evaluate the optical
system?<o:p></o:p></P>
<P class=MsoNormal><o:p> </o:p></P>
<P class=MsoNormal><o:p> </o:p></P>
<P class=MsoNormal>Thanks<o:p></o:p></P>
<P class=MsoNormal><o:p> </o:p></P>
<P class=MsoNormal>Peter<o:p></o:p></P>
<P class=MsoNormal><o:p></o:p></P>
<P class=MsoNormal><o:p> </o:p></P>
<P class=MsoNormal><SPAN style="FONT-FAMILY: Consolas; FONT-SIZE: 10.5pt">Peter
E. Prevelige Jr.<o:p></o:p></SPAN></P>
<P class=MsoNormal><SPAN
style="FONT-FAMILY: Consolas; FONT-SIZE: 10.5pt">Professor<o:p></o:p></SPAN></P>
<P class=MsoNormal><SPAN style="FONT-FAMILY: Consolas; FONT-SIZE: 10.5pt">Dept.
of Microbiology, BBRB 416/6<o:p></o:p></SPAN></P>
<P class=MsoNormal><SPAN style="FONT-FAMILY: Consolas; FONT-SIZE: 10.5pt">Univ.
of Alabama @ Birmingham<o:p></o:p></SPAN></P>
<P class=MsoNormal><SPAN style="FONT-FAMILY: Consolas; FONT-SIZE: 10.5pt">845
19th St. South<o:p></o:p></SPAN></P>
<P class=MsoNormal><SPAN
style="FONT-FAMILY: Consolas; FONT-SIZE: 10.5pt">Birmingham AL.
35294-2170<o:p></o:p></SPAN></P>
<P class=MsoNormal><SPAN style="FONT-FAMILY: Consolas; FONT-SIZE: 10.5pt">Phone
205 975-5327<o:p></o:p></SPAN></P>
<P class=MsoNormal><SPAN style="FONT-FAMILY: Consolas; FONT-SIZE: 10.5pt">FAX
205 975-5479<o:p></o:p></SPAN></P>
<P class=MsoNormal><SPAN style="FONT-FAMILY: Consolas; FONT-SIZE: 10.5pt"><A
href="mailto:prevelig@uab.edu">prevelig@uab.edu</A><o:p></o:p></SPAN></P>
<P class=MsoNormal><SPAN style="FONT-FAMILY: Consolas; FONT-SIZE: 10.5pt"><A
href="http://www.microbio.uab.edu/faculty/prevelige/prevelige-p.htm">http://www.microbio.uab.edu/faculty/prevelige/prevelige-p.htm</A><o:p></o:p></SPAN></P>
<P class=MsoNormal><o:p> </o:p></P></DIV></BODY></HTML>