[RASMB] FW: partial specific volume of RNA

[Cole, James]

Dear Allen-
I've not seen any tables based on nucleotide composition. In any case, it
is dependent on structure and buffer composition so that wouldn't be too
useful. We work a lot with synthetic RNA oligonucleotides and we typically
know the mass.  So we can just  measure the buoyant mass experimentally,
calculate the solvent density based on tabulated data and then calculate
vbar. 

Jim Cole





On 7/12/11 12:54 PM, "Aaron L Lucius" <allucius at uab.edu> wrote:

>I think you can get that information in this paper:
>
>H. Durchschlag , Specific volumes of biological macromolecules and some
>other molecules of biological interest. In: H.-J. Hinz, Editor,
>Thermodynamic Data for Biochemistry and Biotechnology, Springer-Verlag,
>Berlin (1986), pp. 45-128.
>
>However, he references this paper for RNA:  Pearce, T.C., Rowe, A.J.,
>Turnock, G., 1975. Determination of the molecular weights of RNAs by
>low-speed sedimentation equilibrium: 16 S ribosomal RNA as a model
>compound. Journal of molecular biology 97, 193-201.
>
> http://www.sciencedirect.com/science/article/pii/S0022283675800349
>
>Best,
>
>Aaron
>
>Aaron L. Lucius, Ph.D.
>Assistant Professor
>University of Alabama at Birmingham
>Department of Chemistry
>Chem 292 
>1530 3rd Ave S 
>Birmingham, AL 35294-1240
>
>Office: 205-934-8096
>Fax: 205-934-2543
>
>
>-----Original Message-----
>From: rasmb-bounces at server1.bbri.org
>[mailto:rasmb-bounces at server1.bbri.org] On Behalf Of Allen Minton
>Sent: Tuesday, July 12, 2011 11:33 AM
>To: rasmb at server1.bbri.org
>Subject: [RASMB] partial specific volume of RNA
>
>Can anyone provide me with a reference to measurement of the partial
>specific volume of RNA and the possible dependence of partial
>specific volume upon nucleotide composition?
>
>Thanks,
>Allen Minton
>
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