[RASMB] fluorochemicals

[Carlos Alfonso Botello]

Many thanks to Christine EBEL, Claus URBANKE and 
Arne RUFER for their fast reply.
I include their responses that could helpful to other RASMB members.

Carlos

Dear Carlos,
I use Perfluoro-Compound FC-43 ref 12.377.58 of Janssen Chimica
All the best
Christine

Christine EBEL
Institut de Biologie Structurale CEA-CNRS-UJF
41 rue Jules Horowitz, F-38027 Grenoble France
Tel (33) (0) 4 38 78 95 70; Fax (33) (0) 4 38 78 54 94 christine.ebel at ibs.fr
http://www.ibs.fr/content/ibs_eng/presentation/lab/lbm/ebel.htm




Dear Carlos,

the "Fluorinert" oil used as an artificial bottom 
was chemically per-fluorinated tertiary 
tributylamine also known as FC43. It is used in 
mass spectroscopy and can be obtained from 
several sources. I recommend testing it since 
there may be UV absorbing impurities leaking into 
the water phase.  To be on the safe side you may 
incubate it with your buffer before use (maybe at higher temperatures)

Many greetings and best wishes

Claus

Prof. Dr. Claus Urbanke
Am Heuberg 8
29331 Lachendorf

Tel.: 05145 6879
FAX: 05145 284690

Dear Carlos,

you could use Fluorinert FC-40 (Sigma F-9755) or 
Fluorinert FC-77 (Fluka 47090).

Best regards,

Arne





Dr. Arne Rufer
F. Hoffmann-La Roche AG
PROBMO Optical Spectroscopy
065/207
CH-4070 Basel
Tel: 0041-(0)61-6882126
arne.rufer at roche.com





Dr. Carlos Alfonso
Servicio de Ultracentrifuga Analítica
Centro de Investigaciones Biológicas
C/ Ramiro de Maeztu, 9.
28040-MADRID
Tlfno.- 91 8373112 ext.-4297
Fax: 915360432.
E-mail: uanalitica at cib.csic.es
NEW lab web page:
<http://www.cib.csic.es/es/servicio_ultracentrifugacion_analitica>http://www.cib.csic.es/es/servicio_ultracentrifugacion_analitica
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