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Many thanks to Christine EBEL, Claus URBANKE and Arne RUFER for their
fast reply.<br>
I include their responses that could helpful to other RASMB
members.<br><br>
Carlos<br><br>
Dear Carlos,<br>
I use Perfluoro-Compound FC-43 ref 12.377.58 of Janssen Chimica<br>
All the best<br>
Christine<br><br>
Christine EBEL<br>
Institut de Biologie Structurale CEA-CNRS-UJF<br>
41 rue Jules Horowitz, F-38027 Grenoble France<br>
Tel (33) (0) 4 38 78 95 70; Fax (33) (0) 4 38 78 54 94
christine.ebel@ibs.fr<br>
<a href="http://www.ibs.fr/content/ibs_eng/presentation/lab/lbm/ebel.htm" eudora="autourl">
http://www.ibs.fr/content/ibs_eng/presentation/lab/lbm/ebel.htm<br><br>
<br><br>
<br>
</a>Dear Carlos, <br><br>
the "Fluorinert" oil used as an artificial bottom was
chemically per-fluorinated tertiary tributylamine also known as FC43. It
is used in mass spectroscopy and can be obtained from several sources. I
recommend testing it since there may be UV absorbing impurities leaking
into the water phase. To be on the safe side you may incubate it
with your buffer before use (maybe at higher temperatures)<br><br>
Many greetings and best wishes<br><br>
Claus<br><br>
Prof. Dr. Claus Urbanke <br>
Am Heuberg 8<br>
29331 Lachendorf<br><br>
Tel.: 05145 6879<br>
FAX: 05145 284690<br><br>
Dear Carlos,<br><br>
you could use Fluorinert FC-40 (Sigma F-9755) or Fluorinert FC-77 (Fluka
47090).<br><br>
Best regards,<br><br>
Arne<br><br>
<br><br>
<br><br>
Dr. Arne Rufer<br>
F. Hoffmann-La Roche AG<br>
PROBMO Optical Spectroscopy<br>
065/207<br>
CH-4070 Basel<br>
Tel: 0041-(0)61-6882126<br>
arne.rufer@roche.com<br><br>
<br><br>
<br>
<x-sigsep><p></x-sigsep>
Dr. Carlos Alfonso<br>
Servicio de Ultracentrifuga Analítica<br>
Centro de Investigaciones Biológicas<br>
C/ Ramiro de Maeztu, 9. <br>
28040-MADRID<br>
Tlfno.- 91 8373112 ext.-4297 <br>
Fax: 915360432.<br>
E-mail: uanalitica@cib.csic.es<br>
<b>NEW lab web page: <br>
<a href="http://www.cib.csic.es/es/servicio_ultracentrifugacion_analitica">
http://www.cib.csic.es/es/servicio_ultracentrifugacion_analitica<br>
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