[RASMB] Different results with same C(s) model from different softwares

[mitrana at mail.utexas.edu]

Hi Wei Lin

For a reacting system, the peaks do not necessarily correspond to  
individual species except in special circumstances (kinetically  
limited). There is a relatively good online help section along with  
links to the papers on the site where you download SEDFIT.
As for your two peaks in Ultrascan versus one in SEDFIT - if might  
have to do with the regularization in SEDFIT.
-Mitra


Quoting Lee Wei Lin <wlee at imcb.a-star.edu.sg>:

> Hi,
>
> I have done a velocity experiment in hope of doing a quick   
> determination of the number of species and the size of protein   
> complexes formed in my setup.
> I have been doing C(s) analysis using Sedfit and Ultrascan. For most  
>  of my datasets, results generated from both softwares are almost   
> identical, which I expect to be the case. However for 3 of my   
> datasets within the same run, I am getting different results with   
> the two softwares. With Sedfit, I get a single peak at S=3-4. With   
> Ultrascan, I get two peaks at 3.3 and 4.2. Which one should I   
> believe? Do I have 2 species or just 1 species in my sample?
>
> Many thanks in advance.
>
> Wei Lin
>
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