[RASMB] Different results with same C(s) model from different softwares

[Lee Wei Lin]

Hi,

I have done a velocity experiment in hope of doing a quick determination of the number of species and the size of protein complexes formed in my setup.
I have been doing C(s) analysis using Sedfit and Ultrascan. For most of my datasets, results generated from both softwares are almost identical, which I expect to be the case. However for 3 of my datasets within the same run, I am getting different results with the two softwares. With Sedfit, I get a single peak at S=3-4. With Ultrascan, I get two peaks at 3.3 and 4.2. Which one should I believe? Do I have 2 species or just 1 species in my sample?

Many thanks in advance.

Wei Lin

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