[RASMB] SAUCE - x-ray optics for the AUC

[Steve Harding]

Dear Olwyn/Tom/Nick
Just to reinforce what Arthur has said if you can get this to work,
presumably by zonal method it would open a lot of doors for the analysis
of mixed systems.
The new generation differential pressure viscometers which can be
coupled onto SEC also means we can pick out intrinsic viscosities in
such systems. 
We have a project starting under the Dorothy Hodgkin industrial
Partnership scheme in May attempting to extend the method of combining
different hydrodynamic parameters for modelling domain orientation of
multi-domain systems like antibodies* to the case of analysis in
mixed/aggregated systems - commonly found after bioprocessing.  We
previously had to rule out x-ray scattering for such systems but your
proposal sounds like a breath of fresh air - I am happy to give it my
strongest support,
All best
Steve
*Lu, Y., Harding, S.E., Michaelsen, T.E., Longman, E., Davis, K.G.,
Ortega, A., Grossmann, J.G. Sandlie, I. and Garcia De La Torre, J,
(2007). "Solution conformation of wild type and mutant IgG3 and IgG4
immunoglobulins using Crystallohydrodynamics: possible implications for
complement activation". Biophys. J., 93, 3733-3744.
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=2084252
 


http://www.nottingham.ac.uk/ncmh
steve.harding at nottingham.ac.uk
Tel: +44(0) 115 951 6148 (fax 6142)
Mob: +44(0) 78110 90635


 


________________________________

	From: rasmb-bounces at rasmb.bbri.org
[mailto:rasmb-bounces at rasmb.bbri.org] On Behalf Of Arthur Rowe
	Sent: 17 March 2009 11:34
	To: Olwyn Byron; rasmb at rasmb-email.bbri.org
	Subject: Re: [RASMB] SAUCE - x-ray optics for the AUC
	
	

		Just a general thought, colleagues - which maybe has
been considered already. But am I correct in thinking that to get
multiple species resolved into a form sufficiently 'pure' for SAXS
analysis you would be using 'zonal' separation i.e. band-forming cells?
		
		Although few folks seem to actually use this approach in
a conventional AUC, tit does in fact work well, as does the SEDFIT
software for analysis of same. You need to avoid working with low MW
solutes, of course. Do I guess that the Spin Analytical CFA instrument
could handle it all OK?
		
		Arthur
		
		
		

	-- 
	*************************
	Arthur Rowe
	Lab at Sutton Bonington
	tel: +44 115 951 6156
	fax: +44 115 951 6157
	*************************
	
	



		Dear RASMB Colleagues,
		
		We have been invited to submit a full bid to the UK
Science and 
		Technologies Facilities Council (STFC) to fund SAUCE - a
hybrid 
		instrument in which an AUC (the Spin Analytical
Centrifugal Fluid 
		Analyser (CFA)) is introduced into the I22 small angle
x-ray 
		scattering (SAXS) beamline at the UK Diamond
synchrotron.
		
		SAUCE will offer the following advantages to the user:
		
		1. Aggregates will be cleared from the sample in situ;
scattering 
		data will be of a higher quality.
		
		2. AUC modality will permit the separation or
significant enrichment 
		of species in an interacting system; scattering curves
(and thence 
		molecular envelopes) will be determined for the
individual species.
		
		3. It will be possible to acquire AUC and SAXS data
simultaneously.
		
		We are writing to you now to ask if you would be
interested in using 
		SAUCE and would accordingly agree to being included in
the list of 
		beneficiaries?
		
		If so, please reply to this e-mail by return (and
certainly before 24 March).
		
		Many thanks and best wishes,
		
		Olwyn Byron (University of Glasgow, UK)
		Tom Laue (University of New Hampshire, US)
		Nick Terrill (Diamond Synchrotron, UK)
		
		_______________________________________________
		RASMB mailing list
		RASMB at rasmb.bbri.org
		http://rasmb.bbri.org/mailman/listinfo/rasmb
		

	
	
	

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