[RASMB] SAUCE - x-ray optics for the AUC

[Tom Laue]

Hi Arthur, et al.-
The CFA certainly can handle acquiring data from zonal centerpieces. The 
zonal centerpieces have a lot of applications for fluorescecnce, too.
Best wishes,
Tom

Arthur Rowe wrote:
>
>     Just a general thought, colleagues - which maybe has been
>     considered already. But am I correct in thinking that to get
>     multiple species resolved into a form sufficiently 'pure' for SAXS
>     analysis you would be using 'zonal' separation i.e. band-forming
>     cells?
>
>     Although few folks seem to actually use this approach in a
>     conventional AUC, tit does in fact work well, as does the SEDFIT
>     software for analysis of same. You need to avoid working with low
>     MW solutes, of course. Do I guess that the Spin Analytical CFA
>     instrument could handle it all OK?
>
>     Arthur
>
>
> -- 
> *************************
> Arthur Rowe
> Lab at Sutton Bonington
> tel: +44 115 951 6156
> fax: +44 115 951 6157
> *************************
>
>
>
>     Dear RASMB Colleagues,
>
>     We have been invited to submit a full bid to the UK Science and
>     Technologies Facilities Council (STFC) to fund SAUCE - a hybrid
>     instrument in which an AUC (the Spin Analytical Centrifugal Fluid
>     Analyser (CFA)) is introduced into the I22 small angle x-ray
>     scattering (SAXS) beamline at the UK Diamond synchrotron.
>
>     SAUCE will offer the following advantages to the user:
>
>     1. Aggregates will be cleared from the sample in situ; scattering
>     data will be of a higher quality.
>
>     2. AUC modality will permit the separation or significant enrichment
>     of species in an interacting system; scattering curves (and thence
>     molecular envelopes) will be determined for the individual species.
>
>     3. It will be possible to acquire AUC and SAXS data simultaneously.
>
>     We are writing to you now to ask if you would be interested in using
>     SAUCE and would accordingly agree to being included in the list of
>     beneficiaries?
>
>     If so, please reply to this e-mail by return (and certainly before
>     24 March).
>
>     Many thanks and best wishes,
>
>     Olwyn Byron (University of Glasgow, UK)
>     Tom Laue (University of New Hampshire, US)
>     Nick Terrill (Diamond Synchrotron, UK)
>
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Department of Biochemistry and Molecular Biology
University of New Hampshire
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