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<DIV dir=ltr align=left><SPAN class=153392910-18032009>Dear
Olwyn/Tom/Nick</SPAN></DIV>
<DIV dir=ltr align=left><SPAN class=153392910-18032009>Just to reinforce what
Arthur has said if you can get this to work, presumably by zonal method it would
open a lot of doors for the analysis of mixed systems.</SPAN></DIV>
<DIV dir=ltr align=left><SPAN class=153392910-18032009>The new generation
differential pressure viscometers which can be coupled onto SEC also means we
can pick out intrinsic viscosities in such systems. </SPAN></DIV>
<DIV dir=ltr align=left><SPAN
class=153392910-18032009>We have a project starting under the
Dorothy Hodgkin industrial Partnership scheme in May attempting to extend the
method of combining different hydrodynamic parameters for modelling domain
orientation of multi-domain systems like antibodies* to the case of
analysis in mixed/aggregated systems - commonly found after bioprocessing.
We previously had to rule out x-ray scattering for such systems but your
proposal sounds like a breath of fresh air - I am happy to give it my strongest
support,</SPAN></DIV>
<DIV dir=ltr align=left><SPAN class=153392910-18032009>All best</SPAN></DIV>
<DIV dir=ltr align=left><SPAN class=153392910-18032009>Steve</SPAN></DIV>
<DIV dir=ltr align=left><SPAN class=153392910-18032009>*<FONT face=Verdana
size=2>Lu, Y., Harding, S.E., Michaelsen, T.E., Longman, E., Davis, K.G.,
Ortega, A., Grossmann, J.G. Sandlie, I. and Garcia De La Torre, J, (2007).
"Solution conformation of wild type and mutant IgG3 and IgG4 immunoglobulins
using Crystallohydrodynamics: possible implications for complement activation".
Biophys. J., 93, 3733-3744. <A
href="http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=2084252">http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=2084252</A></FONT></SPAN></DIV>
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<P><FONT size=2><A
href="http://www.nottingham.ac.uk/ncmh">http://www.nottingham.ac.uk/ncmh</A><BR>steve.harding@nottingham.ac.uk<BR>Tel:
+44(0) 115 951 6148 (fax 6142)<BR>Mob: +44(0) 78110 90635<BR></FONT></P>
<DIV> </DIV><BR>
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<FONT face=Tahoma size=2><B>From:</B> rasmb-bounces@rasmb.bbri.org
[mailto:rasmb-bounces@rasmb.bbri.org] <B>On Behalf Of </B>Arthur
Rowe<BR><B>Sent:</B> 17 March 2009 11:34<BR><B>To:</B> Olwyn Byron;
rasmb@rasmb-email.bbri.org<BR><B>Subject:</B> Re: [RASMB] SAUCE - x-ray optics
for the AUC<BR></FONT><BR></DIV>
<DIV></DIV>
<BLOCKQUOTE>Just a general thought, colleagues - which maybe has been
considered already. But am I correct in thinking that to get multiple
species resolved into a form sufficiently 'pure' for SAXS analysis you would
be using 'zonal' separation i.e. band-forming cells?<BR><BR>Although few
folks seem to actually use this approach in a conventional AUC, tit does in
fact work well, as does the SEDFIT software for analysis of same. You need
to avoid working with low MW solutes, of course. Do I guess that the Spin
Analytical CFA instrument could handle it all
OK?<BR><BR>Arthur<BR><BR><BR></BLOCKQUOTE>--
<BR>*************************<BR>Arthur Rowe<BR>Lab at Sutton
Bonington<BR>tel: +44 115 951 6156<BR>fax: +44 115 951
6157<BR>*************************<BR><BR>
<BLOCKQUOTE><BR></BLOCKQUOTE><BR>
<BLOCKQUOTE><TT>Dear RASMB Colleagues,<BR><BR>We have been invited to submit
a full bid to the UK Science and <BR>Technologies Facilities Council (STFC)
to fund SAUCE - a hybrid <BR>instrument in which an AUC (the Spin Analytical
Centrifugal Fluid <BR>Analyser (CFA)) is introduced into the I22 small angle
x-ray <BR>scattering (SAXS) beamline at the UK Diamond
synchrotron.<BR><BR>SAUCE will offer the following advantages to the
user:<BR><BR>1. Aggregates will be cleared from the sample in situ;
scattering <BR>data will be of a higher quality.<BR><BR>2. AUC modality will
permit the separation or significant enrichment <BR>of species in an
interacting system; scattering curves (and thence <BR>molecular envelopes)
will be determined for the individual species.<BR><BR>3. It will be possible
to acquire AUC and SAXS data simultaneously.<BR><BR>We are writing to you
now to ask if you would be interested in using <BR>SAUCE and would
accordingly agree to being included in the list of
<BR>beneficiaries?<BR><BR>If so, please reply to this e-mail by return (and
certainly before 24 March).<BR><BR>Many thanks and best wishes,<BR><BR>Olwyn
Byron (University of Glasgow, UK)<BR>Tom Laue (University of New Hampshire,
US)<BR>Nick Terrill (Diamond Synchrotron,
UK)<BR><BR>_______________________________________________<BR>RASMB mailing
list<BR>RASMB@rasmb.bbri.org<BR>http://rasmb.bbri.org/mailman/listinfo/rasmb<BR></TT></BLOCKQUOTE><TT><BR></TT><BR>
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