[RASMB] "wavy" interference optics residuals

[Zou, Qin]

Debra,

If you use Sedfit, show the temperature plot to see if there is a
significant temperature fluctuation.

 

Qin (Chin)  ZOU, Ph.D.

Pfizer Inc.

________________________________

From: rasmb-bounces at rasmb.bbri.org [mailto:rasmb-bounces at rasmb.bbri.org]
On Behalf Of Debbie Eckert
Sent: Thursday, July 16, 2009 11:43 AM
To: rasmb at rasmb.bbri.org
Subject: [RASMB] "wavy" interference optics residuals

 

Hi All,

We have been experiencing a problem with our interference data in which
after data fitting, the residuals frequently have a systematic "wavy"
look to them where they rise above and below zero multiple times >7
across the fitted data.  Subtracting a water blank does not fix the
problem, and it has occurred an several different samples.  In real
time, the fringes also "jump" during centrifugation.  Is this an
indication of an equipment problem, perhaps that there is something
unstable in the centrifuge and/or optical system that is leading to the
movement of the fringe pattern detected by the camera?  If so, is there
a likely culprit?  If not, what is likely to cause this?

Thanks for the help, 

Debra M. Eckert, Ph.D.

Research Assistant Professor of Biochemistry

University of Utah

deckert at biochem.utah.edu

 

 

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