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<p class=MsoNormal><font size=2 color=navy face=Arial><span style='font-size:
10.0pt;font-family:Arial;color:navy'>Debra,<o:p></o:p></span></font></p>
<p class=MsoNormal><font size=2 color=navy face=Arial><span style='font-size:
10.0pt;font-family:Arial;color:navy'>If you use Sedfit, show the temperature
plot to see if there is a significant temperature fluctuation.<o:p></o:p></span></font></p>
<p class=MsoNormal><font size=2 color=navy face=Arial><span style='font-size:
10.0pt;font-family:Arial;color:navy'><o:p> </o:p></span></font></p>
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<p class=MsoNormal><font size=2 color=navy face=Arial><span lang=DE
style='font-size:10.0pt;font-family:Arial;color:navy'>Qin (Chin) ZOU,
Ph.D.</span></font><font color=navy><span lang=DE style='color:navy'><o:p></o:p></span></font></p>
<p class=MsoNormal><font size=2 color=navy face=Arial><span style='font-size:
10.0pt;font-family:Arial;color:navy'>Pfizer Inc.</span></font><font color=navy><span
style='color:navy'><o:p></o:p></span></font></p>
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<p class=MsoNormal><b><font size=2 face=Tahoma><span style='font-size:10.0pt;
font-family:Tahoma;font-weight:bold'>From:</span></font></b><font size=2
face=Tahoma><span style='font-size:10.0pt;font-family:Tahoma'>
rasmb-bounces@rasmb.bbri.org [mailto:rasmb-bounces@rasmb.bbri.org] <b><span
style='font-weight:bold'>On Behalf Of </span></b>Debbie Eckert<br>
<b><span style='font-weight:bold'>Sent:</span></b> Thursday, July 16, 2009
11:43 AM<br>
<b><span style='font-weight:bold'>To:</span></b> rasmb@rasmb.bbri.org<br>
<b><span style='font-weight:bold'>Subject:</span></b> [RASMB] "wavy"
interference optics residuals</span></font><o:p></o:p></p>
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<p class=MsoNormal><font size=3 face="Times New Roman"><span style='font-size:
12.0pt'><o:p> </o:p></span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'>Hi All,<o:p></o:p></span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'>We have been experiencing a problem with our interference
data in which after data fitting, the residuals frequently have a systematic
“wavy” look to them where they rise above and below zero multiple
times >7 across the fitted data. Subtracting a water blank does not
fix the problem, and it has occurred an several different samples. In
real time, the fringes also “jump” during centrifugation. Is
this an indication of an equipment problem, perhaps that there is something unstable
in the centrifuge and/or optical system that is leading to the movement of the
fringe pattern detected by the camera? If so, is there a likely
culprit? If not, what is likely to cause this?<o:p></o:p></span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'>Thanks for the help, <o:p></o:p></span></font></p>
<p class=MsoAutoSig><font size=3 face="Times New Roman"><span style='font-size:
12.0pt'>Debra M. Eckert, Ph.D.<o:p></o:p></span></font></p>
<p class=MsoAutoSig><font size=3 face="Times New Roman"><span style='font-size:
12.0pt'>Research Assistant Professor of Biochemistry<o:p></o:p></span></font></p>
<p class=MsoAutoSig><st1:place w:st="on"><st1:PlaceType w:st="on"><font size=3
face="Times New Roman"><span style='font-size:12.0pt'>University</span></font></st1:PlaceType>
of <st1:PlaceName w:st="on">Utah</st1:PlaceName></st1:place><o:p></o:p></p>
<p class=MsoAutoSig><font size=3 face="Times New Roman"><span style='font-size:
12.0pt'>deckert@biochem.utah.edu<o:p></o:p></span></font></p>
<p class=MsoAutoSig><font size=3 face="Times New Roman"><span style='font-size:
12.0pt'><o:p> </o:p></span></font></p>
<p class=MsoNormal><font size=3 face="Times New Roman"><span style='font-size:
12.0pt'><o:p> </o:p></span></font></p>
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