[RASMB] "wavy" interference optics residuals

[Debbie Eckert]

Hi All,

We have been experiencing a problem with our interference data in which
after data fitting, the residuals frequently have a systematic "wavy" look
to them where they rise above and below zero multiple times >7 across the
fitted data.  Subtracting a water blank does not fix the problem, and it has
occurred an several different samples.  In real time, the fringes also
"jump" during centrifugation.  Is this an indication of an equipment
problem, perhaps that there is something unstable in the centrifuge and/or
optical system that is leading to the movement of the fringe pattern
detected by the camera?  If so, is there a likely culprit?  If not, what is
likely to cause this?

Thanks for the help, 

Debra M. Eckert, Ph.D.

Research Assistant Professor of Biochemistry

University of Utah

deckert at biochem.utah.edu

 

 

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