[RASMB] floatation

[Antje Voelkel]

Dear Matthew,

you have to fill negative s values in the parameter box.
I always run g*(s) before, also negative s values in the parameter box to find the range.

I remember, there was a short description on the analyticalultracentrifugation homepage in FAQ.

best regards
Antje



Hicks, Matthew schrieb:
>
> Hi,
> After returning to AUC after a break of a couple of years I carried 
> out a sedimentation velocity experiment with a peptide in 100% TFE. I 
> did the experiment before realising that since vbar*rho was >1 then 
> the peptide floated (so I guess its a floatation velocity experiment). 
> Presumably one can still analyse the data in the same way with 
> 1-vbar*rho being negative??
> I'm trying to use sedfit (continuous c(s)) but get an error message 
> "log:DOMAIN error".
> Can one use sedfit for floatation and has anyone here done this.
> Thanks for your time,
> Matt
>
> Dr Matthew R. Hicks
> Department of Chemistry
> University of Warwick
> Coventry
> CV4 7AL
> Telephone: +44(0)24 765 23293
> Fax:       +44(0)24 765 24112
>
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-- 

===============================================================

Antje Voelkel
Max-Planck-Institute of Colloids and Interfaces
Colloid Chemistry
Am Muehlenberg 1
14476 Golm
Germany

Tel: +49 331 567 -9528/ -9531
Fax: +49 331 567-9502