[RASMB] k_off rate

[Allen Minton]

If memory serves, many if not most protein association reactions have 
very similar bimolecular association rate constants (say within a 
factor of 3 or so).  I seem to remember something on the order of 
10^5 mol^-1 sec^-1, but I'm not sure of that, you'd better look it 
up.  (Note: this is orders of magnitude smaller than the upper limit 
calculated according to Smoluchowski, which does not take into 
account the orientational constraints applying to a specific 
association that greatly increase the entropic barrier to complex 
formation.)  Therefore, given the equilibrium dissociation constant 
you should be able to estimate the dissociation rate constant to 
within this level of uncertainty.

Diffusion coefficients are irrelevant.

Allen Minton


At 07:59 PM 4/5/2008, Wenta Nikola wrote:
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>Dear Colleagues!
>In order to make my question more precise, I want to to calculate 
>the theoretically diffusion-controlled maximum possible k_off rate 
>of a given dimer based it's kD (from SE run), in combination with a 
>calculated diffusion coefficient for monomer and dimer by HydroPro.
>If anyone had any suggestions how to calculate that k_off rate, I 
>would be very grateful.
>Niko
>
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