[RASMB] Multiple wavelength data

[John Burgner]

If I am collecting data at 280 and above 400nm, I run with the lever
parallel to the stem. This is with the filter out of the beam.  The filter
is for removing  stray light from higher order diffractions from the uv part
of the spectrum and consequently filters out most if not all of the uv
energy.  I usually don't bother with the filter unless I am running a sample
with a high absorbance in the visible range and not collecting data in the
uv. The other problem with the filter is that I usually forget to take it
out of the beam and end up having to stop and restart the centrifuge, which
is annoying to say the least.
John   

-----Original Message-----
From: rasmb-bounces at rasmb.bbri.org [mailto:rasmb-bounces at rasmb.bbri.org] On
Behalf Of Gu-Gang Chang
Sent: Tuesday, April 10, 2007 9:50 PM
To: RASMB at server1.bbri.org
Subject: [RASMB] Multiple wavelength data 

Hi

I have a simple technical question.  Can I collect UV (<400 nm) and Visible
(>400 nm) data simultaneously for the same sample? There is a blocking
filter in the XL-A monochromator.  If I want collect AUC data at two
wavelengths 280 nm and 410 nm, which position should I choose? Parallel or
perpendicular to stem?

Thanks
ggc


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