[RASMB] pH stability

[Virgil Schirf]

Dear Yasuaki,

The Beckman compatibility tables list a 1% NaOH solution as satisfactory
in epon-charcoal filled centerpieces, but not anodized aluminum.
Kristian Schilling from Nanolytics offers titanium 2-channel and
6-channel centerpieces that are chemically inert and resistant to most
alkali and acidic conditions and would be useful in these types of
experiments. If these peptides/proteins require high speeds for adequate
resolution, you also have to consider that epon-charcoal, while
chemically compatible, may not be compatible for the required speed. If
you are reading these samples at 280 nm, I would choose sapphire windows
for their durability and resistance properties. I agree with Dmitry that
the fill hole screws and the aluminum housing may present a problem,
especially if leakage occurs. Proper insulation with the polyethylene
gasket may help prevent any problems, but there are no guarantees.

Sincerely,

Virgil

Yasuaki Hiromasa wrote:

> Dear RASMB members,
> I am working with peptide/protein at basic condition. I want to
> characterize it at pH10-12 or 1-10 mM NaOH. Can I apply under these
> condition to Analytical ultracentrifuge? Will these base conditions
> hurt the cell assembly?
> We have aluminum and epon centerpieces. Windows are quartz and sapphire.
> We want to do velocity and equilibrium experiments.
> Has anybody experience at these conditions?
>
> Sincerely,
> Yasuaki HIromasa
> Department od Biochemistry
> Kansas State University
>
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-- 

Virgil Schirf, Manager CAUMA & CLS
Department of Biochemistry, Rm 4.523U
The University of Texas Health Science Center
7703 Floyd Curl Dr.
San Antonio TX 78229-3900
Voicemail: 210 567-6590
Fax: 210 567-6595
Email: schirfv at biochem.uthscsa.edu