[RASMB] AUC with RNA

[Virgil Schirf]

Hi Dror,

The most obvious consideration is the removal or RNases from the cells 
and other plasticware, etc that will come into contact with the sample. 
We had success doing an equilibrium experiment whereby we soaked the 
windows and centerpieces in 1 M HCl for 2 hours, rinsed with DEPC 
treated water, soaked again with 1 M NaOH for 2 hours, rinsed again with 
DEPC treated water and rinsed with methanol before assembling the cell. 
The pipet tips and tubes were autoclaved for 45 minutes. Another method 
is to treat the individual pieces with a commercial RNase inhibitor such 
as RNaseZap available from Ambion according to their directions.

Good luck and Happy Holidays to all,

Virgil

Dror Noy wrote:

> Hi All,
> A colleague has interested me in a project that may involve AUC  
> measurements of RNA. I have good experience with measuring proteins  
> but none with RNA. I'd greatly appreciate any information, through  
> this forum or directly to my email, about working with RNA in the  
> analytical ultracentrifuge particularly any special considerations  
> that are different from protein work.
> Thanks,
> Dror
>
>
>
> =========================
> Dror Noy, Ph.D.
> Plant Sciences Dept.
> Weizmann Institute of Science
> Rehovot 76100
> Israel
>
> e-mail: dror.noy at weizmann.ac.il
> Tel: (972)-8-934 4300
> Fax: (972)-8-934 4184
>
>
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> RASMB mailing list
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> http://rasmb.bbri.org/mailman/listinfo/rasmb
>

-- 

Virgil Schirf, Manager CAUMA & CLS
Department of Biochemistry, Rm 4.523U
The University of Texas Health Science Center
7703 Floyd Curl Dr.
San Antonio TX 78229-3900
Voicemail: 210 567-6590
Fax: 210 567-6595
Email: schirfv at biochem.uthscsa.edu