[RASMB] Can I get ls-g*(s) without meniscus and RI noise fitting?

[Peter Schuck]

Yi-Ming,
usually I do not fit with RI noise when analyzing absorbance data, unless 
there is something in the buffer that makes the absorbance change with 
time.   Regarding the fitting range, you have to always adjust this to 
exclude optical artifacts in the meniscus region from the data to be 
considered in the analysis.  In your case of an ls-g*(s) analysis, you also 
want to exclude the back-diffusion region (since ls-g*(s) won't be able to 
describe that).
Peter


At 02:05 PM 3/2/2006, Yi-Ming_Li at hgsi.com wrote:
>Hi:
>Using Sedit, I am trying to get the ls-g*(s) profiles of some proteins from
>the absorbance data. I found that fitting the meniscus and RI noise
>results in the baseline going up in low s region. It seems that fitting
>range is also important for the shape of the ls-g*(s). So I would like to
>know if it is ok to get the ls-g*(s) without meniscus floating (fitting)
>and RI noise fitting and set the data fitting range starting point (6.1500
>cm) a little bit further away from the meniscus (5.9771 cm).
>
>Thanks!
>
>Yi-Ming
>
>
>
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