[RASMB] Working with glycerol solutions

[Tom Laue]

Hi Jo-
Both S and D are affected by the same amount, assuming that there is no 
glycerol-dependent changes in oligomerization of the protein.
The time to reach equilibrium is much longer, so it is useful to use 
shorter solution columns if possible (e.g. cutting the column height in 
half will reduce the equilibration time by a factor of four).
There will be a slight density and viscosity gradient in the solution 
due to the redistribution of the glycerol. I believe Sedfit can take 
this into account.
Best wishes,
Tom

pjgb at mrc-lmb.cam.ac.uk wrote:

> Dear RASMBers,
>
> Has anyone had experience of working with significant percentages of 
> glycerol (10-25%), for either velocity or equilibrium runs?
> Obviously the main problem is going to be the greatly increased 
> viscosity, particularly at 5°C with 20°C less of a problem, but what 
> other snags are there?  Also, does the theoretical reduced diffusion, 
> at these high viscosities, work out in practice so that the boundaries 
> are no broader after spinning for the extra time needed?
>
> Jo
>
> P.J.G. Butler,
> MRC Laboratory of Molecular Biology,
> Hills Road, Cambridge, CB2 2QH, UK.
> Tel. +44 (0)1223 402296
> _______________________________________________
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> RASMB at rasmb.bbri.org
> http://rasmb.bbri.org/mailman/listinfo/rasmb
>

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