[RASMB] Working with glycerol solutions

[pjgb at mrc-lmb.cam.ac.uk]

Dear RASMBers,

Has anyone had experience of working with significant percentages of 
glycerol (10-25%), for either velocity or equilibrium runs?
Obviously the main problem is going to be the greatly increased viscosity, 
particularly at 5°C with 20°C less of a problem, but what other snags are 
there?  Also, does the theoretical reduced diffusion, at these high 
viscosities, work out in practice so that the boundaries are no broader 
after spinning for the extra time needed?

Jo

P.J.G. Butler,
MRC Laboratory of Molecular Biology,
Hills Road, Cambridge, CB2 2QH, UK.
Tel. +44 (0)1223 402296