[RASMB] triton removal

[EJ Longman]

Does anyone have any advice on removal of triton X100 from protein samples? 
There seem to be a number of commercially available systems specifically 
for triton removal (as dialysis and gel filtration are not effective).

I have purified two proteins (molecular weights of  30 & 40 kDa) with 0.1% 
triton in the buffers. The triton is not necessary for the stability of the 
proteins but improves the purity of the protein obtained. I would like to 
remove it as its high absorbance will cause problems in my AUC experiments.

I am also interested to know whether detergent removal can be efficient 
enough that afterwards the sample can be used for AUC without having to 
worry about density matching?

Thanks,

Emma


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EJ Longman
biejl at bristol.ac.uk