[RASMB] memb. prot. prep. appl.
Ariel Lustig
ariel.lustig at bluewin.ch
Tue Jul 4 03:29:36 PDT 2006
Dear Colleauges,
Sorry boring you again (since yesterday).
Actually the (3rd) comment, but this time mostly for membrane-protein interessted.
In a new Journal LAB TIMES 2 -2006 in english, I think for european only?( the same publisher who issues
Laborjournal in german) is a column " tips and tricks". At the last part (see attachment) are some remarks for preparative centrifugation, that until now I never saw explecit commented, I mean for the case of
hydrated detergent-micelles and complexes.
The use of Vertical-preparative rotors for bouyant density sucrose or CsCl gradiens (is similar to analytical rotor/cell size) helps if the density bands knowladge acieved from analytical centrifugation for all porposes
not only in case of membrane-proteins.
A nice summer to you all.....ariel
PS
sorry that the pdf the yournal sent me (attachment) are more than page 46.
nothing to du with my subject
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