FW: [RASMB] DMSO

[Cole, James]

Hi Norbert
I've done  some sedimentation equilibrium experiments in charcoal filled
epon cells looking at the effects of small molecule inhibitors  on
protein oligomerization where the drugs were dissolved in DMSO.  It was
a long time ago and I don't have access to my old notebooks from Merck,
but I believe  I went up to  at least 2% DMSO. There was no effect on
the centerpieces. 
Jim 

  _____  

From: rasmb-bounces at server1.bbri.org
[mailto:rasmb-bounces at server1.bbri.org] On Behalf Of John Philo
Sent: Thursday, March 23, 2006 10:44 AM
To: rasmb at server1.bbri.org
Subject: RE: [RASMB] DMSO


Norbert,
 
I'm pretty sure 0.5% DMSO is no problem with Epon, but the person who
would know for certain is Natalia Markova at Biovitrum in Switzerland.
She looks at small molecules that come out of drug screens and their
binding to proteins and I believe she has extensively studied various
issues regarding DMSO and its effects on proteins. Since she hasn't yet
responded I suspect she is not on the RASMB though so I will respond to
you privately with her contact information.
 
John

	-----Original Message-----
	From: rasmb-bounces at rasmb.bbri.org
[mailto:rasmb-bounces at rasmb.bbri.org] On Behalf Of Norbert Muecke
	Sent: Thursday, March 23, 2006 5:36 AM
	To: rasmb at server1.bbri.org
	Subject: [RASMB] DMSO
	
	
	Dear RASMBs,
	we do have a solution with 0.5% DMSO.
	Is this a problem with standard Epon chacoal-filled cells?
	In the manual from Beckman it is written that DMSO interact with
these cells.

	Will it be a problem in the Aluminium cells? Or with the
gaskets? Or housing plugs?
	We do have this problem now and a fast answer would be very
helpful.

	Thank you in advance
	Norbert

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