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<DIV dir=ltr align=left><FONT face=Arial color=#0000ff size=2><SPAN
class=449302816-23032006>Hi Norbert</SPAN></FONT></DIV>
<DIV dir=ltr align=left><FONT face=Arial color=#0000ff size=2><SPAN
class=449302816-23032006>I've done some sedimentation equilibrium
experiments in charcoal filled epon cells looking at the effects of small
molecule inhibitors on protein oligomerization where the drugs were
dissolved in DMSO. It was a long time ago and I don't have access to my
old notebooks from Merck, but I believe I went up to at least 2%
DMSO. There was no effect on the centerpieces. </SPAN></FONT></DIV>
<DIV dir=ltr align=left><FONT><SPAN class=449302816-23032006></SPAN></FONT><SPAN
class=449302816-23032006></SPAN><FONT face=Arial><FONT color=#0000ff><FONT
size=2>J<SPAN class=449302816-23032006>im </SPAN></FONT></FONT></FONT><BR></DIV>
<DIV class=OutlookMessageHeader lang=en-us dir=ltr align=left>
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<FONT face=Tahoma size=2><B>From:</B> rasmb-bounces@server1.bbri.org
[mailto:rasmb-bounces@server1.bbri.org] <B>On Behalf Of </B>John
Philo<BR><B>Sent:</B> Thursday, March 23, 2006 10:44 AM<BR><B>To:</B>
rasmb@server1.bbri.org<BR><B>Subject:</B> RE: [RASMB] DMSO<BR></FONT><BR></DIV>
<DIV></DIV>
<DIV>
<DIV><SPAN class=194512815-23032006><FONT face=Arial color=#0000ff
size=2>Norbert,</FONT></SPAN></DIV>
<DIV><SPAN class=194512815-23032006><FONT face=Arial color=#0000ff
size=2></FONT></SPAN> </DIV>
<DIV><SPAN class=194512815-23032006><FONT face=Arial><FONT color=#0000ff><FONT
size=2>I'm pretty sure 0.5% DMSO is no problem with Epon, but the person who
would know for certain is Natalia Markova at Biovitrum in Switzerland. She looks
at small molecules<SPAN class=680253815-23032006> that come out of drug screens
and their </SPAN>binding to proteins and <SPAN class=680253815-23032006>I
believe she </SPAN>has extensively studied various issues regarding DMSO<SPAN
class=680253815-23032006> and its effects on proteins</SPAN>.<SPAN
class=680253815-23032006> Since she hasn't yet responded I suspect she is not on
the RASMB though so I will respond to you privately with her contact
information.</SPAN></FONT></FONT></FONT></SPAN></DIV>
<DIV><SPAN class=194512815-23032006><FONT face=Arial><FONT color=#0000ff><FONT
size=2><SPAN
class=680253815-23032006></SPAN></FONT></FONT></FONT></SPAN> </DIV>
<DIV><SPAN class=194512815-23032006><FONT face=Arial><FONT color=#0000ff><FONT
size=2><SPAN
class=680253815-23032006>John</SPAN></FONT></FONT></FONT></SPAN></DIV></DIV>
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<DIV class=OutlookMessageHeader lang=en-us dir=ltr align=left><FONT
face=Tahoma size=2>-----Original Message-----<BR><B>From:</B>
rasmb-bounces@rasmb.bbri.org [mailto:rasmb-bounces@rasmb.bbri.org] <B>On
Behalf Of </B>Norbert Muecke<BR><B>Sent:</B> Thursday, March 23, 2006 5:36
AM<BR><B>To:</B> rasmb@server1.bbri.org<BR><B>Subject:</B> [RASMB]
DMSO<BR><BR></FONT></DIV>
<DIV align=left><FONT face=Arial><SPAN style="FONT-SIZE: 10pt">Dear
RASMBs,</SPAN></FONT></DIV>
<DIV align=left><FONT face=Arial><SPAN style="FONT-SIZE: 10pt">we do have a
solution with 0.5% DMSO.</SPAN></FONT></DIV>
<DIV align=left><FONT face=Arial><SPAN style="FONT-SIZE: 10pt">Is this a
problem with standard Epon chacoal-filled cells?</SPAN></FONT></DIV>
<DIV align=left><FONT face=Arial><SPAN style="FONT-SIZE: 10pt">In the manual
from Beckman it is written that DMSO interact with these
cells.</SPAN></FONT></DIV>
<DIV align=left><BR></DIV>
<DIV align=left><FONT face=Arial><SPAN style="FONT-SIZE: 10pt">Will it be a
problem in the Aluminium cells? Or with the gaskets? Or housing
plugs?</SPAN></FONT></DIV>
<DIV align=left><FONT face=Arial><SPAN style="FONT-SIZE: 10pt">We do have this
problem now and a fast answer would be very helpful.</SPAN></FONT></DIV>
<DIV align=left><BR></DIV>
<DIV align=left><FONT face=Arial><SPAN style="FONT-SIZE: 10pt">Thank you in
advance</SPAN></FONT></DIV>
<DIV align=left><FONT face=Arial><SPAN
style="FONT-SIZE: 10pt">Norbert</SPAN></FONT></DIV>
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