[RASMB] meniscus matching centerpiece
Ariel Lustig
ariel.lustig at bluewin.ch
Wed Jan 18 13:28:37 PST 2006
Hi Michael Stoner and interested,
I hope I understood your question !!
When I worked with the >> DELTA S' mode<< (differential sedimentation) we srcatched a capillary between the two sectors , similar to the capillary of a double-sector-syntetic-boundary cell, just on the lowest possible place.
To compensate the air another scratch on top the sectors needed. We used an Epon-Alum centerpiece.
The size of the capillary depends on the viscosity of the solutions you like to match.
Worthwhile to do it in a workshop where the operator has some magnification glass and sharp scratcher.
We tested it with water and sugar water. usualy after few rpm's the miniscii are matched.
A probleme may be if no contact should occur between the two solutions, then we added first in each sector 20 or more mL of FC-43 ,(Flouro-carbo fluid )Beckman sells this product= ro about 1.7gr/mL and it does not mix with water , but with many organic solvents (I have a list). Befor really use we made a lot of pre-runs for test. Sometime
the capillary bloks by some dirt , then we open the centerpiece and rescratch a bit the capillary.
Sure that on this way you sacrify a good centerpiece.....ariel
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