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<DIV><FONT face=Arial size=2>Hi Michael Stoner and interested,</FONT></DIV>
<DIV><FONT face=Arial size=2>I hope I understood your question
!!</FONT></DIV>
<DIV><FONT face=Arial size=2>When I worked with the
>> DELTA S' mode<< (differential sedimentation) we
srcatched a capillary between </FONT><FONT face=Arial size=2>the two
sectors , similar to the capillary of a double-sector-syntetic-boundary cell,
just on the lowest possible place. </FONT></DIV>
<DIV><FONT face=Arial size=2>To compensate the air another
scratch on top the sectors needed. We used an Epon-Alum
centerpiece.</FONT></DIV>
<DIV><FONT face=Arial size=2>The size of the capillary depends on the
viscosity of the solutions you like to match. </FONT></DIV>
<DIV><FONT face=Arial size=2>Worthwhile to do it in a workshop where the
operator has some magnification glass and sharp scratcher.</FONT></DIV>
<DIV><FONT face=Arial size=2>We tested it with water and sugar water.
usualy after few rpm's the miniscii are matched. </FONT></DIV>
<DIV><FONT face=Arial size=2>A probleme may be if no contact should
occur between the two solutions, then we added first in each sector 20 or
more mL of FC-43 ,(Flouro-carbo fluid )Beckman sells this product= ro
about 1.7gr/mL and it does not mix with water , but with many
organic solvents (I have a list). Befor really use we made a lot of
pre-runs for test. Sometime </FONT></DIV>
<DIV><FONT face=Arial size=2>the capillary bloks by some dirt , then
we open the centerpiece and rescratch a bit the capillary.</FONT></DIV>
<DIV><FONT face=Arial size=2>Sure that on this way you sacrify a
good centerpiece.....ariel</FONT></DIV></BODY></HTML>