[RASMB] lower molecular weight

[Beld, Joris]

Dear all,

Some time ago I asked around for the highest measurable molecular weight by analytical ultracentrifugation. This time a colleague asked me whether it is possible to measure host-guest complexes by analytical ultracentrifugation in which the host is around 1500 Da and the guest around 500 Da. I checked literature on this more supramolecular chemistry related research (in contrast to the more biochemical research normally done by AU) and so far without any luck. Could someone help me with a lead? To me it seems to be possible, although one needs to spin the sample pretty fast. Apart from this question: what would be the experiment of choice? Sedimentation velocity or sedimentation equilibrium?
Thanks!
Kind regards,

Joris Beld
Swiss Federal Institute of Technology
Hilvert Group