[RASMB] how to determine base of cell

[ospivey at bmb-fs1.biochem.okstate.edu]

Dear Karl,

    I suspect the methods suggested by others is the best approach, 
but I did ask Beckman for the manufacturing values several years ago. 
These are: 6.174, 6.675, and 7.177 cm for the bottoms of the A, B, 
and C sectors, respectively,

Olin Spivey




At 11:47 AM +0000 11/27/03, pjgb at mrc-lmb.cam.ac.uk wrote:
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>Dear Karl,
>
>An Intensity scan should show you the relevant information - though 
>you may well have the complication that the 2 sectors for each 
>position do not have exactly identical radii, due to slight 
>alignment errors (or even manufacturing tolerances).
>
>Jo
>
>--On Wednesday, November 26, 2003 13:03 -0700 Karl.Maluf at UCHSC.edu wrote:
>
>>
>>Dear RASMB-ers,
>>
>>I am performing a series of sedimentation equilibrium experiments using
>>the 6 cell centerpieces.  For my analysis, I need to determine accurately
>>the radial position that corresponds to the bottom of each chamber in the
>>centerpiece.  It seems straightforward to determine the miniscus of each
>>chamber, but I am not sure of the best way to go about determining the
>>bottom of each chamber.  Any help, and especially any literature
>>references will be greatly appreciated.
>>N. Karl Maluf
>>U. of Colorado Health Sciences Center
>>Denver, CO.
>
>
>
>P.J.G. Butler,
>MRC Laboratory of Molecular Biology,
>Hills Road, Cambridge, CB2 2QH, UK.
>Tel. +44 (0)1223 402296
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-- 
H. Olin Spivey
246 NRC
Dept. Biochem. & Molec. Biol.
phone: 405 744-6192
fax: 405 744-7799
email: ospivey at biochem.okstate.edu