[RASMB] how to determine base of cell

[pjgb at mrc-lmb.cam.ac.uk]

Dear Karl,

An Intensity scan should show you the relevant information - though you may 
well have the complication that the 2 sectors for each position do not have 
exactly identical radii, due to slight alignment errors (or even 
manufacturing tolerances).

Jo

--On Wednesday, November 26, 2003 13:03 -0700 Karl.Maluf at UCHSC.edu wrote:

>
> Dear RASMB-ers,
>
> I am performing a series of sedimentation equilibrium experiments using
> the 6 cell centerpieces.  For my analysis, I need to determine accurately
> the radial position that corresponds to the bottom of each chamber in the
> centerpiece.  It seems straightforward to determine the miniscus of each
> chamber, but I am not sure of the best way to go about determining the
> bottom of each chamber.  Any help, and especially any literature
> references will be greatly appreciated.
> N. Karl Maluf
> U. of Colorado Health Sciences Center
> Denver, CO.



P.J.G. Butler,
MRC Laboratory of Molecular Biology,
Hills Road, Cambridge, CB2 2QH, UK.
Tel. +44 (0)1223 402296